Linked Life Data

7506249

Source:http://linkedlifedata.com/resource/pubmed/id/7506249

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-2-7
pubmed:abstractText
Electron-probe analysis provides an objective criterion for the physiological status of cells: whether they show the high potassium and low sodium that are expected of healthy animal cells. Preparing isolated goldfish hair cells that were healthy by this criterion required several precautions, including: limited exposure to enzymes and to simple salt solutions, a rest period between enzyme treatment and mechanical disruption of the tissue, and presence of bovine albumin in the medium both during the rest period and during mechanical dispersion and plating. Cells prepared with these precautions from the saccule and lagena and kept in an enriched medium had the following elemental composition (mole percentages with respect to phosphorus): K, 103; Na, 18; Cl, 23; S, 13; Mg, 8; Ca, 1.5. These mole percentages were close to these elements' total millimolar concentrations in the cells. If the precautions were not taken, cells with intact surface membranes (as assessed by exclusion and retention of dyes) could be obtained, but the cells had elevated cell sodium and low cell potassium.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0378-5955
pubmed:author
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9-21
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Electron-probe analysis of isolated goldfish hair cells: implications for preparing healthy cells.
pubmed:affiliation
Eaton-Peabody Laboratory, Massachusetts Eye and Ear Infirmary, Boston 02114.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't