Linked Life Data

7505843

Source:http://linkedlifedata.com/resource/pubmed/id/7505843

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1994-1-28
pubmed:abstractText
We have previously demonstrated that retrovirus-mediated genes were transferred to mouse glioma cells in a meningeal gliomatosis model (Yamada et al.: Japanese Journal of Cancer Research 83:1244-1247, 1992). This retrovirus vector contains the Escherichia coli. beta-galactosidase (beta-gal) gene as a marker for integration of the lacZ gene, which is controlled by the SV40 early promoter. We investigated whether lacZ genes could be specifically controlled in mouse glioma cells by glial-specific promoters, including the 2.5 kb 5' flanking region of the mouse glial fibrillary acidic protein (GFAP) gene, the 1.3 kb 5' flanking region of the myelin basic protein (MBP) gene, and the 1.5 kb 5' flanking region of the myelin proteolipid protein (PLP) gene. Psi-2 packaging cells were transfected with each retrovirus vector (GFAP promoter-, MBP promoter-, and PLP promoter-lacZ) and the infectious virus particles were recovered from the supernatants. Blue staining for beta-gal was detected in various fibroblast, myeloma, and glioma cell lines transduced with the retrovirus BAG vector. On the other hand, blue staining was only detected in glioma cells after transduction with the lacZ gene-bearing retrovirus controlled by glial-specific promoters. The strongest promoter activity was detected after transduction with the retrovirus in which the MBP promoter controlled the lacZ gene. Mouse glioma cells transduced with retrovirus containing the MBP promoter directing the herpes simplex virus type 1 thymidine kinase (HTK) gene were extremely sensitive to ganciclovir, while the parental cells and cells transduced with retrovirus containing the lacZ gene were not sensitive to ganciclovir.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0360-4012
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
472-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:7505843-3T3 Cells, pubmed-meshheading:7505843-Animals, pubmed-meshheading:7505843-Escherichia coli, pubmed-meshheading:7505843-Ganciclovir, pubmed-meshheading:7505843-Gene Expression, pubmed-meshheading:7505843-Gene Therapy, pubmed-meshheading:7505843-Genetic Vectors, pubmed-meshheading:7505843-Glial Fibrillary Acidic Protein, pubmed-meshheading:7505843-Glioma, pubmed-meshheading:7505843-Herpesvirus 1, Human, pubmed-meshheading:7505843-Mice, pubmed-meshheading:7505843-Mice, Inbred C3H, pubmed-meshheading:7505843-Mice, Inbred C57BL, pubmed-meshheading:7505843-Myelin Basic Proteins, pubmed-meshheading:7505843-Myelin Proteins, pubmed-meshheading:7505843-Myelin Proteolipid Protein, pubmed-meshheading:7505843-Promoter Regions, Genetic, pubmed-meshheading:7505843-Retroviridae, pubmed-meshheading:7505843-Simian virus 40, pubmed-meshheading:7505843-Thymidine Kinase, pubmed-meshheading:7505843-Transfection, pubmed-meshheading:7505843-Tumor Cells, Cultured, pubmed-meshheading:7505843-beta-Galactosidase
pubmed:year
1993
pubmed:articleTitle
Selective expression of foreign genes in glioma cells: use of the mouse myelin basic protein gene promoter to direct toxic gene expression.
pubmed:affiliation
Department of Neurosurgery, Osaka University Medical School, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't