Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
1994-1-21
pubmed:abstractText
Estimation of gene number in mammals is difficult due to the high proportion of noncoding DNA within the nucleus. In this study, we provide a direct measurement of the number of genes in human and mouse. We have taken advantage of the fact that many mammalian genes are associated with CpG islands whose distinctive properties allow their physical separation from bulk DNA. Our results suggest that there are approximately 45,000 CpG islands per haploid genome in humans and 37,000 in the mouse. Sequence comparison confirms that about 20% of the human CpG islands are absent from the homologous mouse genes. Analysis of a selection of genes suggests that both human and mouse are losing CpG islands over evolutionary time due to de novo methylation in the germ line followed by CpG loss through mutation. This process appears to be more rapid in rodents. Combining the number of CpG islands with the proportion of island-associated genes, we estimate that the total number of genes per haploid genome is approximately 80,000 in both organisms.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-1301997, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-1336093, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-1338771, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-1351867, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-1365911, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-1379743, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-1387107, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-1505946, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-1546979, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-1574125, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-1631131, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-1838348, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-1974172, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-2129226, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-2173146, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-2352943, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-2445562, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-2549255, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-2667218, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-2777259, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-2981636, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-3034483, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-3595568, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-3627985, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-3656447, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-6188105, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-6253937, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-6279862, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-6287455, http://linkedlifedata.com/resource/pubmed/commentcorrection/7505451-6996571
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11995-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Number of CpG islands and genes in human and mouse.
pubmed:affiliation
Institute of Cell and Molecular Biology, University of Edinburgh, Scotland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't