Linked Life Data

7505083

Source:http://linkedlifedata.com/resource/pubmed/id/7505083

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1994-1-25
pubmed:abstractText
This paper describes a DNA/nucleolar organizer region-associated protein (NOR) double staining technic and quantitation method. On smeared cell slides, the acidic proteins associated with nucleolar organizer regions were revealed by the silver colloid technic, and DNA was stained with the fluorescent dye Hoechst 33342. The simultaneous quantitation of silver stained proteins (AgNORs) and DNA was performed with image cytometry. Two applications of this method are presented: a study of AgNOR expression during the MCF-7 cell line cycle and the simultaneous quantitation of DNA and AgNORs in non-Hodgkin's lymphoma node imprints. This method offers the advantage of evaluating, on the same cell at the same time, the DNA content (ploidy) and AgNOR expression, which constitute a good approximation of the cell's proliferation state. Since ploidy and proliferation index are being used more and more in tumor assessment, it is useful to obtain them in minimum time and with a minimum of biologic material.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0884-6812
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
351-7
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Simultaneous quantitation of DNA and nucleolar organizer regions by image cytometry.
pubmed:affiliation
Equipe de Cytologie Quantitative, Laboratoire de Génétique, Histologie et Biologie de la Reproduction, Faculté de Médecine de Grenoble, France.
pubmed:publicationType
Journal Article