Linked Life Data

7504636

Source:http://linkedlifedata.com/resource/pubmed/id/7504636

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-1-7
pubmed:abstractText
Extracellular matrix proteins were presented in culture to postmitotic, epithelial precursors of chick lens fiber cells as rigid, planar surfaces or malleable gels. Their ability to maintain and promote differentiated characteristics was judged by tritiated thymidine incorporation, immunologic detection of a cytokeratin-like protein (CP49) which accumulates during lens fiber development, and the formation of multicellular aggregates known as lentoids. Regardless of their composition, planar substrates stimulated the reentry into the cell cycle by promoting cell spreading. Laminin- and type IV collagen-coated surfaces facilitated monolayer growth and no appreciable accumulation of CP49. Thin films of Matrigel, which initially stimulated cell division, eventually promoted the formation of extensive lentoids, multicellular aggregates exhibiting many morphologic and biochemical properties of lens fibers. Malleable gels of Matrigel, however, inhibited cell division and immediately allowed the cells to begin lentoid formation. Culture conditions which favored lentoid formation also showed greatly enhanced levels of CP49 accumulation. In addition, lentoids were also shown to accumulate an integral membrane protein (MIP26) which is present in communicating junctions between neighboring fiber cells. These studies indicate that increased cell associations within forming lentoids may influence the progression of lens fiber terminal differentiation in vitro.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0012-1606
pubmed:author
pubmed:issnType
Print
pubmed:volume
160
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
494-503
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Cell-cell interactions affect the accumulation of a cytokeratin-like protein during lens fiber development.
pubmed:affiliation
Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan 48201.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't