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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1994-1-4
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pubmed:abstractText |
A monoclonal antibody to the LamB protein, named LBS-1, was developed and characterized. It was then covalently bound to Sepharose and used to purify hybrid LamB proteins from Escherichia coli crude extracts. A peptide of the interferon-gamma (IFN-gamma) NH2-terminal region, inserted within the LamB protein, was used as a model to assess immune response in mice injected with sonicated E. coli extract or with affinity-purified hybrid LamB protein. None of the mice immunized with the whole bacterial extract produced antibodies to IFN-gamma. On the other hand, all the mice immunized with the purified protein developed high-titre anti-IFN-gamma antibodies. These results might be due to the presence of bacterial components capable of masking the LamB protein to the immune system. The use of affinity-purified LamB proteins may constitute in some instances a more effective way of generating an immune response against foreign epitopes as opposed to whole bacterial antigens.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0264-410X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1093-6
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:7504378-Amino Acid Sequence,
pubmed-meshheading:7504378-Animals,
pubmed-meshheading:7504378-Antibodies, Monoclonal,
pubmed-meshheading:7504378-Antibody Formation,
pubmed-meshheading:7504378-Antibody Specificity,
pubmed-meshheading:7504378-Bacterial Outer Membrane Proteins,
pubmed-meshheading:7504378-Bacterial Vaccines,
pubmed-meshheading:7504378-Base Sequence,
pubmed-meshheading:7504378-Epitopes,
pubmed-meshheading:7504378-Escherichia coli,
pubmed-meshheading:7504378-Female,
pubmed-meshheading:7504378-Hybridomas,
pubmed-meshheading:7504378-Immunization,
pubmed-meshheading:7504378-Interferon-gamma,
pubmed-meshheading:7504378-Mice,
pubmed-meshheading:7504378-Mice, Inbred BALB C,
pubmed-meshheading:7504378-Molecular Sequence Data,
pubmed-meshheading:7504378-Porins,
pubmed-meshheading:7504378-Receptors, Virus
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pubmed:year |
1993
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pubmed:articleTitle |
High-titre antibodies to a foreign epitope elicited by affinity-purified hybrid LamB proteins.
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pubmed:affiliation |
Institute of Microbiology, University of Brescia, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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