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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1994-1-5
|
| pubmed:abstractText |
To investigate the electrophysiological effects of the stimulation of the metabotropic excitatory amino acid receptors, we applied trans-1-amino-cyclopentane-1,3-dicarboxylate, an agonist of this type of receptors, on presumed rat dopamine cells intracellularly recorded in vitro. Trans-1-amino-cyclopentane-1,3-dicarboxylate (3-30 microM, t-ACPD) caused a sustained increase of the spontaneous firing rate and a depolarization. When the membrane potential was held at about the resting level (-50, -60 mV), by the single-electrode voltage-clamp technique, t-ACPD induced an inward current. In 57% of the tested cells the inward current was associated with a decrease of the apparent input conductance. In the remaining cells no obvious changes in membrane conductance were observed. The active form of t-ACPD, (1S,3R)-1-amino-cyclopentane-1,3-dicarboxylate [3-50 microM, (1S,3R)-ACPD] also produced a reversible inward current on the dopaminergic cells and this was antagonized by (S)-4-carboxy-3-hydroxyphenylglycine (300 microM), a selective antagonist of the (1S,3R)-ACPD-induced depolarization on central neurons. The (1S,3R)-ACPD-induced inward current was not antagonized by L-2-amino-3-phosphonopropionic acid (100 microM), an antagonist of the t-ACPD-induced activation of inositide synthesis. 6-cyano-7-nitroquinoxaline-2,3-dione (10 microM), an alfa-amino-3-hydroxy-5- methyl-isoxazole propionic acid/kainate antagonist, DL-amino-5-phosphonopentanoic acid (30 microM), an N-methyl-D-aspartate antagonist, and scopolamine (10 microM), a muscarinic antagonist, did not significantly affect the actions of t-ACPD. A block of synaptic transmission obtained by applying tetrodotoxin failed to prevent the action of t-ACPD.(ABSTRACT TRUNCATED AT 250 WORDS)
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-amino-1,3-dicarboxycyclopentane,
http://linkedlifedata.com/resource/pubmed/chemical/2-amino-3-phosphonopropionic acid,
http://linkedlifedata.com/resource/pubmed/chemical/4-carboxy-3-hydroxyphenylglycine,
http://linkedlifedata.com/resource/pubmed/chemical/6-Cyano-7-nitroquinoxaline-2,3-dione,
http://linkedlifedata.com/resource/pubmed/chemical/Alanine,
http://linkedlifedata.com/resource/pubmed/chemical/Cycloleucine,
http://linkedlifedata.com/resource/pubmed/chemical/Glycine,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Quinoxalines,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Metabotropic Glutamate,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrodotoxin,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-Amino-3-hydroxy-5-methyl-4-iso...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0306-4522
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
56
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
399-407
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| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7504216-6-Cyano-7-nitroquinoxaline-2,3-dione,
pubmed-meshheading:7504216-Action Potentials,
pubmed-meshheading:7504216-Alanine,
pubmed-meshheading:7504216-Animals,
pubmed-meshheading:7504216-Cycloleucine,
pubmed-meshheading:7504216-Dopamine,
pubmed-meshheading:7504216-Electrophysiology,
pubmed-meshheading:7504216-Female,
pubmed-meshheading:7504216-Glycine,
pubmed-meshheading:7504216-Male,
pubmed-meshheading:7504216-Neurons,
pubmed-meshheading:7504216-Potassium,
pubmed-meshheading:7504216-Quinoxalines,
pubmed-meshheading:7504216-Rats,
pubmed-meshheading:7504216-Rats, Wistar,
pubmed-meshheading:7504216-Receptors, Metabotropic Glutamate,
pubmed-meshheading:7504216-Stimulation, Chemical,
pubmed-meshheading:7504216-Tetrodotoxin,
pubmed-meshheading:7504216-alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Activation of metabotropic glutamate receptors induces an inward current in rat dopamine mesencephalic neurons.
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| pubmed:affiliation |
Dip. Sanità Pubblica, Università di Roma Tor Vergata, Italy.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|