rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
11
|
pubmed:dateCreated |
1996-1-16
|
pubmed:abstractText |
Influenza NS2 protein was expressed in Saccharomyces cerevisiae using a copper-inducible promoter. The protein produced had a molecular weight of 13 kDa, was reactive with anti-NS2 antiserum and was localised to the yeast cell nucleus. Two-hybrid analysis identified a direct protein-protein interaction between NS2 and the M2 protein of the virus, involving the C-terminal 163 residues of M1. A filter-binding assay localised the M1 binding region to the C-terminal 70 amino acids of NS2.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:issn |
0304-8608
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
140
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
2067-73
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:7503702-Amino Acid Sequence,
pubmed-meshheading:7503702-Base Sequence,
pubmed-meshheading:7503702-Cloning, Molecular,
pubmed-meshheading:7503702-DNA Primers,
pubmed-meshheading:7503702-Influenza A virus,
pubmed-meshheading:7503702-Molecular Sequence Data,
pubmed-meshheading:7503702-Protein Binding,
pubmed-meshheading:7503702-Recombinant Proteins,
pubmed-meshheading:7503702-Saccharomyces cerevisiae,
pubmed-meshheading:7503702-Viral Matrix Proteins,
pubmed-meshheading:7503702-Viral Nonstructural Proteins
|
pubmed:year |
1995
|
pubmed:articleTitle |
Expression and analysis of the NS2 protein of influenza A virus.
|
pubmed:affiliation |
Biomolecular Research Institute, Parkville, Victoria, Australia.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|