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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
1996-1-17
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pubmed:abstractText |
A set of three 14mer oligodeoxyribonucleotides of sequence d(5'-CTATGGCCTCAG*CT-3'/3'-GATACCGGAGTCGA-5') containing G* variants either as 2'-deoxyguanosine phosphate (unmodified), N7-cyanoborane 2'-deoxyguanosine phosphate (base-modified) or 2'-deoxyguanosine boranophosphate (backbone-modified) were synthesized by template-directed primer extension. Both the N7-cyanoborane 2'-deoxyguanosine triphosphate and 2'-deoxyguanosine alpha-boranotriphosphate nucleotides are good substrates for Sequenase. We infer that a single Sp boranophosphate linkage (which has a stereochemistry equivalent to the corresponding Rp thiophosphate analog) is formed in the backbone-modified 14mer. Thermally induced helix-coil transitions were monitored for the hybridized duplexes using UV and circular dichroism (CD) spectroscopy. The CD spectra of the two types of boron-modified hybrids closely resemble the unmodified parent duplex, forming B-type helices in 150 mM NaCl, 1 mM EDTA, 10 mM phosphate, pH 7.4, buffer. UV melting results indicate that both hybrids have stabilities comparable with the parent duplex as measured by Tm or delta G degree 25. These studies indicate that singly modified base- or backbone-boronated DNA are good analogs of normal DNA.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-1630898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-1711677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-1741280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-2011517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-224029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-2322546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-2411211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-2765487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-2836790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-2836793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-3264723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-3499613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-3601658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-4687151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-6200155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-6261793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-6824629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-6850059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-7013804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-7045112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-7260332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-7470481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-8029016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-8036171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-8117931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-8127651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-8242139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-8312256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501475-843596
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4495-501
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7501475-Base Sequence,
pubmed-meshheading:7501475-Boranes,
pubmed-meshheading:7501475-Circular Dichroism,
pubmed-meshheading:7501475-Deoxyguanosine,
pubmed-meshheading:7501475-Molecular Sequence Data,
pubmed-meshheading:7501475-Nucleic Acid Conformation,
pubmed-meshheading:7501475-Nucleic Acid Denaturation,
pubmed-meshheading:7501475-Oligodeoxyribonucleotides,
pubmed-meshheading:7501475-Spectrophotometry, Ultraviolet,
pubmed-meshheading:7501475-Thermodynamics
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pubmed:year |
1995
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pubmed:articleTitle |
Boron-containing oligodeoxyribonucleotide 14mer duplexes: enzymatic synthesis and melting studies.
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pubmed:affiliation |
Department of Chemistry, Duke University, Durham, NC 27708-0346, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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