| pubmed-article:7501441 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7501441 | lifeskim:mentions | umls-concept:C0016055 | lld:lifeskim |
| pubmed-article:7501441 | lifeskim:mentions | umls-concept:C0022984 | lld:lifeskim |
| pubmed-article:7501441 | lifeskim:mentions | umls-concept:C0028948 | lld:lifeskim |
| pubmed-article:7501441 | lifeskim:mentions | umls-concept:C0380603 | lld:lifeskim |
| pubmed-article:7501441 | lifeskim:mentions | umls-concept:C0085862 | lld:lifeskim |
| pubmed-article:7501441 | lifeskim:mentions | umls-concept:C1299583 | lld:lifeskim |
| pubmed-article:7501441 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:7501441 | lifeskim:mentions | umls-concept:C1608386 | lld:lifeskim |
| pubmed-article:7501441 | lifeskim:mentions | umls-concept:C1549571 | lld:lifeskim |
| pubmed-article:7501441 | lifeskim:mentions | umls-concept:C0210233 | lld:lifeskim |
| pubmed-article:7501441 | lifeskim:mentions | umls-concept:C0599533 | lld:lifeskim |
| pubmed-article:7501441 | pubmed:issue | 21 | lld:pubmed |
| pubmed-article:7501441 | pubmed:dateCreated | 1996-1-17 | lld:pubmed |
| pubmed-article:7501441 | pubmed:abstractText | Antisense oligodeoxynucleotides can selectively inhibit the expression of individual genes and thus have potential applications in anticancer and antiviral therapy. A critical prerequisite to their use as therapeutic agents is the understanding of their non-specific interactions with biological structures, e.g. proteins. In this study we examined the interactions of P-chiral phosphorothioate oligodeoxynucleotides with several proteins. The Rp- and Sp- diastereomers, and racemic machine-made mixtures, or M-oligodeoxynucleotides were used independently as competitors of the binding of a probe, phosphodiester oligodeoxynucleotide bearing a 5' alkylating moiety, to rsCD4, bFGF and laminin. These oligodeoxynucleotides were also used as competitors of the binding of a non-alkylating probe M-phosphorothioate oligodeoxynucleotide, 5'-32P-SdT18 to fibronectin. The average values of and quantitative estimates for the IC50 of competition and the constant of competition (Kc) of Rp-, Sp- and M-stereoisomers of several homo- and heteropolymer oligodeoxynucleotides were determined and compared. Surprisingly, in the proteins we studied, the values of IC50 and Kc for the Rp-, Sp- and M-oligodeoxynucleotides were essentially identical. Thus, the ability of the phosphorothioate oligodeoxynucleotides we employed, to bind to the proteins studied in this work, is virtually independent of P-chirality. Our results also imply that the role of the purine and pyrimidine bases in oligodeoxynucleotide-protein interactions, as well as the nature of the contact points (sulfur versus oxygen) between the oligomer and the protein, may be relatively unimportant. | lld:pubmed |
| pubmed-article:7501441 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7501441 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7501441 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7501441 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7501441 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:7501441 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:7501441 | pubmed:author | pubmed-author:SteinC ACA | lld:pubmed |
| pubmed-article:7501441 | pubmed:author | pubmed-author:TonkinsonJ... | lld:pubmed |
| pubmed-article:7501441 | pubmed:author | pubmed-author:Koziolkiewicz... | lld:pubmed |
| pubmed-article:7501441 | pubmed:author | pubmed-author:ZeltserRR | lld:pubmed |
| pubmed-article:7501441 | pubmed:author | pubmed-author:GugaPP | lld:pubmed |
| pubmed-article:7501441 | pubmed:author | pubmed-author:KarwowskiBB | lld:pubmed |
| pubmed-article:7501441 | pubmed:author | pubmed-author:StecWW | lld:pubmed |
| pubmed-article:7501441 | pubmed:author | pubmed-author:BenimetskayaL... | lld:pubmed |
| pubmed-article:7501441 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7501441 | pubmed:day | 11 | lld:pubmed |
| pubmed-article:7501441 | pubmed:volume | 23 | lld:pubmed |
| pubmed-article:7501441 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7501441 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7501441 | pubmed:pagination | 4239-45 | lld:pubmed |
| pubmed-article:7501441 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:7501441 | pubmed:meshHeading | pubmed-meshheading:7501441-... | lld:pubmed |
| pubmed-article:7501441 | pubmed:meshHeading | pubmed-meshheading:7501441-... | lld:pubmed |
| pubmed-article:7501441 | pubmed:meshHeading | pubmed-meshheading:7501441-... | lld:pubmed |
| pubmed-article:7501441 | pubmed:meshHeading | pubmed-meshheading:7501441-... | lld:pubmed |
| pubmed-article:7501441 | pubmed:meshHeading | pubmed-meshheading:7501441-... | lld:pubmed |
| pubmed-article:7501441 | pubmed:meshHeading | pubmed-meshheading:7501441-... | lld:pubmed |
| pubmed-article:7501441 | pubmed:meshHeading | pubmed-meshheading:7501441-... | lld:pubmed |
| pubmed-article:7501441 | pubmed:meshHeading | pubmed-meshheading:7501441-... | lld:pubmed |
| pubmed-article:7501441 | pubmed:meshHeading | pubmed-meshheading:7501441-... | lld:pubmed |
| pubmed-article:7501441 | pubmed:meshHeading | pubmed-meshheading:7501441-... | lld:pubmed |
| pubmed-article:7501441 | pubmed:meshHeading | pubmed-meshheading:7501441-... | lld:pubmed |
| pubmed-article:7501441 | pubmed:meshHeading | pubmed-meshheading:7501441-... | lld:pubmed |
| pubmed-article:7501441 | pubmed:meshHeading | pubmed-meshheading:7501441-... | lld:pubmed |
| pubmed-article:7501441 | pubmed:meshHeading | pubmed-meshheading:7501441-... | lld:pubmed |
| pubmed-article:7501441 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7501441 | pubmed:articleTitle | Binding of phosphorothioate oligodeoxynucleotides to basic fibroblast growth factor, recombinant soluble CD4, laminin and fibronectin is P-chirality independent. | lld:pubmed |
| pubmed-article:7501441 | pubmed:affiliation | Columbia University, College of Physicians and Surgeons, New York, NY 10032, USA. | lld:pubmed |
| pubmed-article:7501441 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7501441 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7501441 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7501441 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7501441 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7501441 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7501441 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7501441 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7501441 | lld:pubmed |
