Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
1996-1-18
|
| pubmed:databankReference | |
| pubmed:abstractText |
Surfactant protein-D (SP-D) is a collectin found associated with surfactant in the lung. SP-D has also been functionally characterized as an opsonin for diverse microorganisms and a chemoattractant for phagocytic cells. To determine the structure of mouse SP-D, we isolated and characterized clones from a B6/CBAF1J strain lung cDNA library using a PCR-derived genomic probe. The deduced sequence predicts a 19-amino acid signal sequence, a 25-amino acid long NH2 terminus with two cysteines, followed by an uninterrupted collagen domain with 59 Gly-X-Y repeats. Next, a short "neck" domain of 28 amino acids, with a potential to form trimeric alpha-helical coiled coil is found ending in a COOH-terminal 125-amino acid carbohydrate recognition domain. The mature mouse SP-D protein of 355 amino acids shows strong homology to rat (92% identity), human (76%), and bovine (72%) SP-D amino acid sequences. Northern blot and RT-PCR analysis revealed that the mouse SP-D gene is expressed predominantly in lung and, surprisingly, also in heart, stomach, and kidney but not in brain. In contrast, mouse surfactant protein-A (SP-A) mRNA expression was found to be restricted to lung. Human lung and stomach, but not heart or liver, were found to express SP-D mRNA, as determined by PCR. The mouse SP-D gene (Sftp4) has been localized to chromosome 14 (to a region syntenic to human chromosome 10), closely linked to the genes for other collagenous lectins, mannose-binding protein-A (MbI1), and SP-A (Sftp1).
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactants,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
155
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
5671-7
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7499852-Amino Acid Sequence,
pubmed-meshheading:7499852-Animals,
pubmed-meshheading:7499852-Base Sequence,
pubmed-meshheading:7499852-Chromosome Mapping,
pubmed-meshheading:7499852-Cloning, Molecular,
pubmed-meshheading:7499852-Glycoproteins,
pubmed-meshheading:7499852-Mice,
pubmed-meshheading:7499852-Mice, Inbred C57BL,
pubmed-meshheading:7499852-Mice, Inbred CBA,
pubmed-meshheading:7499852-Molecular Sequence Data,
pubmed-meshheading:7499852-Organ Specificity,
pubmed-meshheading:7499852-Polymerase Chain Reaction,
pubmed-meshheading:7499852-Proteolipids,
pubmed-meshheading:7499852-Pulmonary Surfactant-Associated Protein A,
pubmed-meshheading:7499852-Pulmonary Surfactant-Associated Protein D,
pubmed-meshheading:7499852-Pulmonary Surfactant-Associated Proteins,
pubmed-meshheading:7499852-Pulmonary Surfactants,
pubmed-meshheading:7499852-RNA, Messenger,
pubmed-meshheading:7499852-Sequence Alignment,
pubmed-meshheading:7499852-Sequence Homology, Amino Acid
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Mouse surfactant protein-D. cDNA cloning, characterization, and gene localization to chromosome 14.
|
| pubmed:affiliation |
Department of Pathology, Boston University School of Medicine, MA 02118, USA.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|