Linked Life Data

7499402

Source:http://linkedlifedata.com/resource/pubmed/id/7499402

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
48
pubmed:dateCreated
1996-1-18
pubmed:abstractText
We studied the effect of ionizing radiation on the activation of the AP-1 transcription factors and the regulation of basic fibroblast growth factor (bFGF) gene expression in drug-sensitive human breast carcinoma (MCF-7) cells and its drug-resistant variant (MCF-7/ADR) cells. Northern blot and gel mobility shift assays showed that 135 cGy of ionizing radiation induced c-jun and c-fos gene expression, AP-1 binding activity, as well as bFGF gene expression in MCF-7/ADR cells. In MCF-7 cells, however, we observed little/no induction of bFGF gene expression and AP-1 binding activity after the stress. Nevertheless, MCF-7 cells transfected with plasmids containing c-jun gene contain high levels of bFGF protein. H-7 (60 micrograms/ml), a potent protein kinase C (PKC) inhibitor, inhibited the stress-induced AP-1 binding activity and bFGF gene expression in MCF-7/ADR cells. Corroborating this observation, overexpression of PKC alpha induced bFGF gene expression in MCF-7 cells. Taken together, these results suggest that stress-induced bFGF gene expression is mediated through the activation of PKC and AP-1 transcription factors. Differences in the levels of PKC activity and AP-1 binding factors may be responsible for differential expression of bFGF among breast cancer cell lines. Although there are large differences in response to ionizing radiation between MCF-7 and MCF-7/ADR cell lines, we observed no significant differences in radiocytotoxicity between them.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
270
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
28790-6
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:7499402-1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine, pubmed-meshheading:7499402-Base Sequence, pubmed-meshheading:7499402-Breast Neoplasms, pubmed-meshheading:7499402-Cell Survival, pubmed-meshheading:7499402-Drug Resistance, Multiple, pubmed-meshheading:7499402-Drug Resistance, Neoplasm, pubmed-meshheading:7499402-Enzyme Inhibitors, pubmed-meshheading:7499402-Fibroblast Growth Factor 2, pubmed-meshheading:7499402-Gene Expression Regulation, Neoplastic, pubmed-meshheading:7499402-Genes, fos, pubmed-meshheading:7499402-Genes, jun, pubmed-meshheading:7499402-Humans, pubmed-meshheading:7499402-Isoquinolines, pubmed-meshheading:7499402-Molecular Sequence Data, pubmed-meshheading:7499402-Oligodeoxyribonucleotides, pubmed-meshheading:7499402-Piperazines, pubmed-meshheading:7499402-Protein Binding, pubmed-meshheading:7499402-Protein Kinase C, pubmed-meshheading:7499402-Radiation, Ionizing, pubmed-meshheading:7499402-Transcription Factor AP-1, pubmed-meshheading:7499402-Tumor Cells, Cultured
pubmed:year
1995
pubmed:articleTitle
Effect of ionizing radiation on AP-1 binding activity and basic fibroblast growth factor gene expression in drug-sensitive human breast carcinoma MCF-7 and multidrug-resistant MCF-7/ADR cells.
pubmed:affiliation
Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, Michigan 48073, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't