Linked Life Data

7499401

Source:http://linkedlifedata.com/resource/pubmed/id/7499401

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
48
pubmed:dateCreated
1996-1-18
pubmed:databankReference
pubmed:abstractText
Molecular cDNA cloning, two-dimensional gel immunoblotting, and amino acid microsequencing identified three sequence-unique and distinct proteins that constitute a subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins corresponding to hnRNPs H, H', and F. These proteins share epitopes and sequence identity with two other proteins, isoelectric focusing sample spot numbers 2222 (37.6 kDa; pI 6.5) and 2326 (39.5 kDa; pI 6.6), indicating that the subfamily may contain additional members. The identity between hnRNPs H and H' is 96%, between H and F 78%, and between H' and F 75%, respectively. The three proteins contain three repeats, which we denote quasi-RRMs (qRRMs) since they have a remote similarity to the RNA recognition motif (RRM). The three qRRMs of hnRNP H, with a few additional NH2-terminal amino acids, were constructed by polymerase chain reaction amplification and used for ribohomopolymer binding studies. Each qRRM repeat bound poly(rG), while only the NH2-terminal qRRM bound poly(rC) and poly(rU). None of the repeats bound detectable amounts of poly(rA). The expression levels of hnRNPs H and F were differentially regulated in pairs of normal and transformed fibroblasts and keratinocytes. In normal human keratinocytes, the expression level of H was unaffected by treatment with several substances tested including two second messengers and seven cytokines. Likewise the expression level of F was independent of these substances, although it was strikingly down-regulated by long term treatment with 4 beta-phorbol 12-myristate 13-acetate, indicating that the protein kinase C signaling pathway regulates its expression. No effect of 4 beta-phorbol 12-myristate 13-acetate was observed on the expression of hnRNP H. The genes coding for hnRNPs H, H', and F were chromosome-mapped to 5q35.3 (HNRPH1), 6q25.3-q26, and/or Xq22 (HNRPH2) and 10q11.21-q11.22 (HNRPF), respectively.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
270
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
28780-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7499401-Amino Acid Sequence, pubmed-meshheading:7499401-Animals, pubmed-meshheading:7499401-Base Sequence, pubmed-meshheading:7499401-Cell Line, pubmed-meshheading:7499401-Cells, Cultured, pubmed-meshheading:7499401-Chromosome Mapping, pubmed-meshheading:7499401-Chromosomes, Human, Pair 5, pubmed-meshheading:7499401-Chromosomes, Human, Pair 6, pubmed-meshheading:7499401-DNA, Complementary, pubmed-meshheading:7499401-DNA Primers, pubmed-meshheading:7499401-Epitopes, pubmed-meshheading:7499401-Heterogeneous-Nuclear Ribonucleoprotein Group F-H, pubmed-meshheading:7499401-Heterogeneous-Nuclear Ribonucleoproteins, pubmed-meshheading:7499401-Humans, pubmed-meshheading:7499401-Molecular Sequence Data, pubmed-meshheading:7499401-RNA, Heterogeneous Nuclear, pubmed-meshheading:7499401-RNA-Binding Proteins, pubmed-meshheading:7499401-Ribonucleoproteins, pubmed-meshheading:7499401-Sequence Homology, Amino Acid, pubmed-meshheading:7499401-X Chromosome
pubmed:year
1995
pubmed:articleTitle
Heterogeneous nuclear ribonucleoproteins H, H', and F are members of a ubiquitously expressed subfamily of related but distinct proteins encoded by genes mapping to different chromosomes.
pubmed:affiliation
Danish Centre for Human Genome Research, Department of Medical Biochemistry, University of Aarhus, Denmark.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't