Linked Life Data

7499242

Source:http://linkedlifedata.com/resource/pubmed/id/7499242

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
46
pubmed:dateCreated
1996-1-17
pubmed:databankReference
pubmed:abstractText
We have isolated a cDNA encoding a novel human intracytoplasmic tyrosine kinase, termed RAFTK (for a related adhesion focal tyrosine kinase). In addition, we have cloned and characterized the murine homolog of the human RAFTK cDNA. Comparison of the deduced amino acid sequences of human RAFTK and murine Raftk cDNAs revealed 95% homology, indicating that RAFTK is highly conserved between these species. The RAFTK cDNA clone, encoding a polypeptide of 1009 amino acids, has closest homology (48% identity, 65% similarity) to the focal adhesion kinase (pp125FAK). Comparison of the deduced amino acid sequences also indicates that RAFTK, like pp125FAK, lacks a transmembrane region, myristylation sites, and SH2 and SH3 domains. In addition, like pp125FAK, RAFTK contains a kinase domain flanked by large N-terminal (426 residues) and C-terminal (331 residues) domains, and the C-terminal region contains a predicted proline-rich stretch of residues. In fetal tissues, RAFTK expression was abundant in brain, and low levels were observed in lung and liver. In adult tissues, it was less restricted, indicating that RAFTK expression is developmentally up-regulated. Expression of RAFTK was also observed in human CD34+ marrow cells, primary bone marrow megakaryocytes, platelets, and various areas of brain. The human RAFTK gene was assigned to human chromosome 8 using genomic DNAs from human/rodent somatic cell hybrid lines. The mouse Raftk gene was mapped to chromosome 14, closely linked to gonadotropin-releasing hormone. Using specific antibodies for RAFTK, a approximately 123-kDa protein from the human megakaryocytic CMK cell line was immunoprecipitated. Treatment of the megakaryocytic CMK cells with thrombin caused a rapid induction of tyrosine phosphorylation of RAFTK protein. The structural features of RAFTK suggest that it is a member of the focal adhesion kinase gene family and may participate in signal transduction in human megakaryocytes and brain as well as in other cell types.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
270
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
27742-51
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:7499242-Amino Acid Sequence, pubmed-meshheading:7499242-Animals, pubmed-meshheading:7499242-Base Sequence, pubmed-meshheading:7499242-Bone Marrow, pubmed-meshheading:7499242-Brain, pubmed-meshheading:7499242-Cell Line, pubmed-meshheading:7499242-Cells, Cultured, pubmed-meshheading:7499242-Chromosome Mapping, pubmed-meshheading:7499242-Cloning, Molecular, pubmed-meshheading:7499242-Conserved Sequence, pubmed-meshheading:7499242-Crosses, Genetic, pubmed-meshheading:7499242-DNA, Complementary, pubmed-meshheading:7499242-DNA Primers, pubmed-meshheading:7499242-Fetus, pubmed-meshheading:7499242-Focal Adhesion Kinase 2, pubmed-meshheading:7499242-Gene Expression Regulation, Enzymologic, pubmed-meshheading:7499242-Humans, pubmed-meshheading:7499242-Hybrid Cells, pubmed-meshheading:7499242-Megakaryocytes, pubmed-meshheading:7499242-Mice, pubmed-meshheading:7499242-Mice, Inbred C57BL, pubmed-meshheading:7499242-Molecular Sequence Data, pubmed-meshheading:7499242-Muridae, pubmed-meshheading:7499242-Organ Specificity, pubmed-meshheading:7499242-Polymerase Chain Reaction, pubmed-meshheading:7499242-Protein-Tyrosine Kinases, pubmed-meshheading:7499242-Recombination, Genetic, pubmed-meshheading:7499242-Sequence Homology, Amino Acid
pubmed:year
1995
pubmed:articleTitle
Identification and characterization of a novel related adhesion focal tyrosine kinase (RAFTK) from megakaryocytes and brain.
pubmed:affiliation
Deaconess Hospital, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.