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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1996-1-17
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pubmed:databankReference |
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pubmed:abstractText |
A full-length cDNA encoding the putative hepatic glycogen-binding (GL) subunit of protein phosphatase-1 (PP1) was isolated from a rat liver library. The deduced amino acid sequence (284 residues, 32.6 kDa) was 23% identical (39% similar) to the N-terminal region of the glycogen-binding (GM) subunit of PP1 from striated muscle. The similarities between GM and GL were most striking between residues 63-86, 144-166 and 186-227 of human GM (approximately 40% identity), nearly all the identities with the putative yeast homologue GAC1 being located between 144-166 and 186-227. The cDNA was expressed in E. coli, and the expressed protein transformed the properties of PP1 to those characteristic of the hepatic glycogen-associated enzyme. These experiments establish that the cloned protein is GL.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
375
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
294-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:7498521-Amino Acid Sequence,
pubmed-meshheading:7498521-Animals,
pubmed-meshheading:7498521-Base Sequence,
pubmed-meshheading:7498521-Binding Sites,
pubmed-meshheading:7498521-Carrier Proteins,
pubmed-meshheading:7498521-Cloning, Molecular,
pubmed-meshheading:7498521-DNA Primers,
pubmed-meshheading:7498521-Gene Library,
pubmed-meshheading:7498521-Glutathione Transferase,
pubmed-meshheading:7498521-Humans,
pubmed-meshheading:7498521-Kinetics,
pubmed-meshheading:7498521-Liver,
pubmed-meshheading:7498521-Liver Glycogen,
pubmed-meshheading:7498521-Macromolecular Substances,
pubmed-meshheading:7498521-Molecular Sequence Data,
pubmed-meshheading:7498521-Muscle, Skeletal,
pubmed-meshheading:7498521-Open Reading Frames,
pubmed-meshheading:7498521-Organ Specificity,
pubmed-meshheading:7498521-Phosphoprotein Phosphatases,
pubmed-meshheading:7498521-Phosphorylase a,
pubmed-meshheading:7498521-Polymerase Chain Reaction,
pubmed-meshheading:7498521-Protein Phosphatase 1,
pubmed-meshheading:7498521-Rabbits,
pubmed-meshheading:7498521-Rats,
pubmed-meshheading:7498521-Recombinant Fusion Proteins,
pubmed-meshheading:7498521-Sequence Homology, Amino Acid
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pubmed:year |
1995
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pubmed:articleTitle |
Amino acid sequence and expression of the hepatic glycogen-binding (GL)-subunit of protein phosphatase-1.
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pubmed:affiliation |
Department of Biochemistry, University of Dundee, Scotland, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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