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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
1996-1-17
|
| pubmed:databankReference | |
| pubmed:abstractText |
HTLV-I generally integrates at least one full-length copy in adult T-cell leukemia (ATL) cells. A group of patients without full-length provirus have a unique conserved truncation of the provirus which retains env-pX-3'LTR. Tumor cells of a patient from this group were genetically analyzed. Analysis of the 5' and 3' cellular flanking region adjacent to the provirus suggest that the defective provirus was integrated immediately downstream of a promoter of an unknown cellular gene. The activity of the promoter was weak but was responsive to Tax-like HTLV-I LTR. The provirus may have utilized it as a substitute for the 5'LTR and thus 3'LTR may have become an alternative promoter for the cellular gene, which may give similar viral-cellular interactions to that of general cases with full-length proviruses. Surprisingly, the 3' cellular flanking region which is thought to be controlled originally by the promoter is constitutively expressed specifically in an HTLV-I producing ATL cell line HUT1O2G, in which the corresponding region is not modified by provirus. The detection of this HTLV-I-induced transcript provides a probe to find an HTLV-I inducible unknown cellular gene that may be related to the pathogenesis of ATL.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0014-5793
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
13
|
| pubmed:volume |
375
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
31-6
|
| pubmed:dateRevised |
2006-5-1
|
| pubmed:meshHeading |
pubmed-meshheading:7498474-Adult,
pubmed-meshheading:7498474-Aged,
pubmed-meshheading:7498474-Animals,
pubmed-meshheading:7498474-Base Sequence,
pubmed-meshheading:7498474-Cell Line,
pubmed-meshheading:7498474-Cercopithecus aethiops,
pubmed-meshheading:7498474-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:7498474-Conserved Sequence,
pubmed-meshheading:7498474-Defective Viruses,
pubmed-meshheading:7498474-Genes, env,
pubmed-meshheading:7498474-Human T-lymphotropic virus 1,
pubmed-meshheading:7498474-Humans,
pubmed-meshheading:7498474-Kidney,
pubmed-meshheading:7498474-Leukemia, T-Cell,
pubmed-meshheading:7498474-Middle Aged,
pubmed-meshheading:7498474-Molecular Sequence Data,
pubmed-meshheading:7498474-Plasmids,
pubmed-meshheading:7498474-Proviruses,
pubmed-meshheading:7498474-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:7498474-Restriction Mapping,
pubmed-meshheading:7498474-Transcription, Genetic,
pubmed-meshheading:7498474-Transfection,
pubmed-meshheading:7498474-Tumor Cells, Cultured,
pubmed-meshheading:7498474-Virus Integration
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Analysis of a novel defective HTLV-I provirus and detection of a new HTLV-I-induced cellular transcript.
|
| pubmed:affiliation |
Laboratory of Human Tumer Viruses, Kyoto University, Japan.
|
| pubmed:publicationType |
Journal Article
|