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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0018951,
umls-concept:C0143630,
umls-concept:C0162597,
umls-concept:C0183683,
umls-concept:C0204727,
umls-concept:C0205409,
umls-concept:C0344211,
umls-concept:C1171362,
umls-concept:C1171411,
umls-concept:C1317973,
umls-concept:C1515670,
umls-concept:C1521721,
umls-concept:C1533691
|
| pubmed:issue |
13
|
| pubmed:dateCreated |
1996-1-16
|
| pubmed:abstractText |
Although macrophages account for 70-90% of the adherent cells in mouse long-term bone marrow cultures (LTBMC) and CFU-F colonies, the predominant nonhematopoietic stromal cell is endothelial-like (EL), expressing cytoplasmic collagen IV, laminin, and an antigen recognized by the monoclonal antibody MECA-10. We report the isolation of this stromal cell lineage from primary LTBMC by immunomagnetic cell selection using MECA-10. More than 95% of the cells in the MECA-10-positive fraction are EL cells as judged by morphology, surface staining for MECA-10, cytoplasmic staining for collagen IV, and electrophoretic analysis of MECA-10-positive cells isolated from radiation chimeras. When plated under LTBMC conditions, EL cell monolayers recharged with either wild-type or Sl/Sld marrow support an increased density and number of clonogenic and mature hematopoietic cells in short-term cultures. In accord with this finding, Northern blots of mRNA from unstimulated EL cells demonstrate constitutive expression of Kit ligand (KL). Moreover, in situ two-color immunofluorescence staining for cytoplasmic collagen IV and surface KL suggests that EL cells are the exclusive source of membrane-bound KL in mouse cultures. The ability to isolate EL cells from primary cultures without the need for repeated cell passage or immortalization provides a novel approach to dissecting the molecular basis of stem cell-stromal cell interactions.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0301-472X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
23
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1407-16
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7498370-Animals,
pubmed-meshheading:7498370-Antibodies, Monoclonal,
pubmed-meshheading:7498370-Bone Marrow Cells,
pubmed-meshheading:7498370-Cell Lineage,
pubmed-meshheading:7498370-Cells, Cultured,
pubmed-meshheading:7498370-Collagen,
pubmed-meshheading:7498370-Colony-Forming Units Assay,
pubmed-meshheading:7498370-Connective Tissue Cells,
pubmed-meshheading:7498370-Endothelium,
pubmed-meshheading:7498370-Female,
pubmed-meshheading:7498370-Gene Expression,
pubmed-meshheading:7498370-Hematopoiesis,
pubmed-meshheading:7498370-Immunomagnetic Separation,
pubmed-meshheading:7498370-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:7498370-Male,
pubmed-meshheading:7498370-Mice,
pubmed-meshheading:7498370-Mice, Inbred C3H,
pubmed-meshheading:7498370-Mice, Inbred C57BL,
pubmed-meshheading:7498370-Mice, Mutant Strains,
pubmed-meshheading:7498370-Radiation Chimera,
pubmed-meshheading:7498370-Stem Cell Factor
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Isolation of endothelial-like stromal cells that express Kit ligand and support in vitro hematopoiesis.
|
| pubmed:affiliation |
Division of Hematology/Oncology, University of Texas Southwestern Medical Center, Dallas, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|