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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1996-1-11
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pubmed:abstractText |
The response of normal and chronic myeloid leukemia (CML), CD34+ cells to human macrophage inflammatory protein-1 alpha (MIP-1 alpha or LD78) was assessed. In tritiated thymidine incorporation assays, stem cell factor plus granulocyte-macrophage colony-stimulating factor stimulated thymidine incorporation in normal CD34+ cells was reduced to 72% of control values in the presence of MIP-1 alpha, whereas incorporation by CML CD34+ cells exposed to the same factors was not altered. In clonogenic assays, the presence of MIP-1 alpha gave a level of colony formation that was 71% of control values for normal progenitor cells, whereas for CML CD34+ cells colony formation was enhanced by 25%. These results suggest that, in vitro, CML progenitor cells are relatively refractory to the growth inhibitory effects of MIP-1 alpha. Using flow cytometry, the specific binding of a biotinylated human MIP-1 alpha/avidin fluorescein (FITC) conjugate to normal and CML mononuclear and CD34+ cell populations was quantified. The data indicate that (for both normal and CML CD34+ cells) there was a single population of cells that express cell surface receptors for MIP-1 alpha and this receptor expression was independent of cell cycle status. CML progenitor cells may be refractory to the effects of MIP-1 alpha as a result of events downstream from receptor expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL4,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Inflammatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Monokines,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Chemokine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine,
http://linkedlifedata.com/resource/pubmed/chemical/macrophage inflammatory protein...
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4270-7
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:7492787-Antigens, CD34,
pubmed-meshheading:7492787-Base Sequence,
pubmed-meshheading:7492787-Cell Cycle,
pubmed-meshheading:7492787-Chemokine CCL4,
pubmed-meshheading:7492787-Colony-Forming Units Assay,
pubmed-meshheading:7492787-Cytokines,
pubmed-meshheading:7492787-DNA Primers,
pubmed-meshheading:7492787-Hematopoietic Stem Cells,
pubmed-meshheading:7492787-Humans,
pubmed-meshheading:7492787-Leukemia, Myelogenous, Chronic, BCR-ABL Positive,
pubmed-meshheading:7492787-Macrophage Inflammatory Proteins,
pubmed-meshheading:7492787-Molecular Sequence Data,
pubmed-meshheading:7492787-Monokines,
pubmed-meshheading:7492787-Neoplastic Stem Cells,
pubmed-meshheading:7492787-Receptors, Chemokine,
pubmed-meshheading:7492787-Receptors, Immunologic,
pubmed-meshheading:7492787-Thymidine,
pubmed-meshheading:7492787-Tumor Stem Cell Assay
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pubmed:year |
1995
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pubmed:articleTitle |
Macrophage inflammatory protein-1 alpha receptors are present on cells enriched for CD34 expression from patients with chronic myeloid leukemia.
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pubmed:affiliation |
Department of Biochemistry and Applied Molecular Biology, UMIST, Manchester Royal Infirmary, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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