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rdf:type |
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lifeskim:mentions |
umls-concept:C0003241,
umls-concept:C0003320,
umls-concept:C0020971,
umls-concept:C0036111,
umls-concept:C0037868,
umls-concept:C0086418,
umls-concept:C0205263,
umls-concept:C0229671,
umls-concept:C0332161,
umls-concept:C0442027,
umls-concept:C0748342,
umls-concept:C1185740
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pubmed:issue |
2
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pubmed:dateCreated |
1996-1-11
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pubmed:abstractText |
Induction of immune responses in the reproductive tract will be crucial for a functional gamete antigen-based antifertility vaccine. Here we describe the construction and development of an avirulent Salmonella as an oral vaccine delivery vector to elicit sperm-specific immune responses in reproductive tract secretions. A cDNA sequence encoding the human sperm antigen SP10 was cloned on an asd+vector and expressed to a high level in an avirulent delta cya, delta crp, and delta asd vaccine strain of Salmonella typhimurium. Oral immunization of female BALB/c mice with this recombinant Salmonella elicited high-titer anti-SP10 IgG antibodies in serum and IgA antibodies in vaginal secretions. Anti-SP10 antibody titers could be increased by secondary and tertiary oral administrations of the recombinant Salmonella. Induction of sperm-specific antibodies in the reproductive tract following oral administration of a recombinant Salmonella could lead to the development of a simple, safe, efficient, and easy-to-use antifertility vaccine.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ACRV1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Acrv1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Gonadal Steroid Hormones,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin A,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-3363
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
53
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
462-71
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7492701-Acrosome,
pubmed-meshheading:7492701-Animals,
pubmed-meshheading:7492701-Antibodies,
pubmed-meshheading:7492701-Antigens,
pubmed-meshheading:7492701-Contraception, Immunologic,
pubmed-meshheading:7492701-DNA, Complementary,
pubmed-meshheading:7492701-Female,
pubmed-meshheading:7492701-Gene Expression,
pubmed-meshheading:7492701-Gonadal Steroid Hormones,
pubmed-meshheading:7492701-Humans,
pubmed-meshheading:7492701-Immunization,
pubmed-meshheading:7492701-Immunoglobulin A,
pubmed-meshheading:7492701-Immunoglobulin G,
pubmed-meshheading:7492701-Membrane Proteins,
pubmed-meshheading:7492701-Mice,
pubmed-meshheading:7492701-Mice, Inbred BALB C,
pubmed-meshheading:7492701-Salmonella typhimurium,
pubmed-meshheading:7492701-Vaccines, Synthetic,
pubmed-meshheading:7492701-Vagina
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pubmed:year |
1995
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pubmed:articleTitle |
Oral immunization with attenuated Salmonella expressing human sperm antigen induces antibodies in serum and the reproductive tract.
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pubmed:affiliation |
Department of Biology, Washington University, St. Louis, Missouri 63130, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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