Linked Life Data

7491679

Source:http://linkedlifedata.com/resource/pubmed/id/7491679

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1996-1-3
pubmed:abstractText
In this study, bovine islets were isolated by collagenase digestion and density gradient purification, equilibrated stepwise with 3 M dimethylsulfoxide at 24 degrees C, nucleated at -150 degrees C, slow cooled at 0.25 degrees C/min down to -40 degrees C, and finally stored at -150 degrees C. After variable periods of time, the islets were quickly thawed at 37 degrees C, and dimethylsulfoxide was removed by 0.75 M sucrose. Postthawing recovery was 86 +/- 6% islet equivalents. Histology confirmed the identity and morphological integrity of the islets. Insulin release from the frozen-thawed islets was 0.13 +/- 0.03 microU/is/min at 3.3 mmol/L glucose and increased significantly (0.27 +/- 0.04 microU/is/min, P < 0.05) at 25 mmol/L glucose. Encapsulated, cryopreserved islets reversed hyperglycemia in diabetic mice after 6-8 days following implantation. Therefore, the method described in this paper permitted successful cryopreservation of bovine islets of proven in vitro and in vivo viability.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0041-1337
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
60
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1044-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Cryopreservation of purified bovine islets of Langerhans.
pubmed:affiliation
Cattedra Malattie del Metabolismo, Istituto di Clinica Medica II, Pisa, Italy.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't