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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1996-1-2
|
| pubmed:abstractText |
We measured the translocation of exogenous radiolabeled phospholipid probes from the outer to the inner leaflet in the synaptosomal plasma membranes from the electric organs of Narke japonica, a Japanese marine ray. These radioactive probes can provide a convenient and highly sensitive means of measuring the translocation kinetics of phospholipids between outer and inner leaflets of biomembranes that are available only with low yield [Anzai et al. (1993) Biochim. Biophys. Acta 1151, 69-75]. Translocation kinetics revealed that the behavior of phosphatidylserine (PS), phosphatidylethanolamine (PE), and phosphatidylcholine (PC) differed. PS quickly disappeared from the outer leaflet of the bilayer. The translocation of PE and PC was slow and about 80% of the PE- and PC-probes remained in the outer leaflet at 3 h. These results differ from those of translocation in erythrocyte membranes measured using the same probes, where aminophospholipid translocase translocated both PS and PE from the outer to the inner leaflet of the bilayer, although the rate was faster for PS than for PE. In this study, the translocation was apparently PS-specific, and it was inhibited by SH reagent or intrasynaptosomal ATP-depletion. These findings clearly demonstrate that the translocation is driven by an ATP-dependent protein, which has apparent PS-specificity. We suggest that there is a translocase other than erythrocyte-type aminophospholipid translocase in synaptosomal plasma membranes from N. japonica.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Ethylmaleimide,
http://linkedlifedata.com/resource/pubmed/chemical/Iodoacetamide,
http://linkedlifedata.com/resource/pubmed/chemical/Lipid Bilayers,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Lipids,
http://linkedlifedata.com/resource/pubmed/chemical/Molecular Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylcholines,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylethanolamines,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylserines,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Albumin, Bovine
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0021-924X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
117
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1232-7
|
| pubmed:dateRevised |
2007-12-19
|
| pubmed:meshHeading |
pubmed-meshheading:7490265-Adenosine Triphosphate,
pubmed-meshheading:7490265-Animals,
pubmed-meshheading:7490265-Cell Membrane,
pubmed-meshheading:7490265-Electric Organ,
pubmed-meshheading:7490265-Ethylmaleimide,
pubmed-meshheading:7490265-Iodoacetamide,
pubmed-meshheading:7490265-Kinetics,
pubmed-meshheading:7490265-Lipid Bilayers,
pubmed-meshheading:7490265-Membrane Lipids,
pubmed-meshheading:7490265-Microscopy, Electron,
pubmed-meshheading:7490265-Molecular Probes,
pubmed-meshheading:7490265-Phosphatidylcholines,
pubmed-meshheading:7490265-Phosphatidylethanolamines,
pubmed-meshheading:7490265-Phosphatidylserines,
pubmed-meshheading:7490265-Phospholipids,
pubmed-meshheading:7490265-Serum Albumin, Bovine,
pubmed-meshheading:7490265-Skates (Fish),
pubmed-meshheading:7490265-Synaptosomes
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Phosphatidylserine-specific transbilayer lipid translocation in synaptosomal plasma membranes from Narke japonica.
|
| pubmed:affiliation |
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|