Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-1-4
pubmed:abstractText
We have investigated the ability of a constitutively active Gq-alpha mutant, Q209L-alpha q, to regulate target gene expression. Transient expression in GH3 pituitary cells of a rat proximal prolactin promoter-chloramphenicol acetyltransferase construct (-187)PRL-CAT, was stimulated by co-expression of Q209L alpha q, but not by wild-type alpha q. Q209L-alpha q stimulated expression of constructs driven by promoters for either rat prolactin or growth hormone, but not of a control construct driven by the thymidine kinase promoter. Thus, transcriptional effects of alpha q are specific both for the activated state of this G-alpha subunit and the promoter examined. Since both the prolactin and growth hormone promoters are activated by the pituitary cell-specific transcription factor Pit-1, we examined whether a Pit-1 binding site could direct a response to Q209L-alpha q. Two copies of prolactin promoter Pit-1 binding site 1P conferred upon a heterologous metallothionein promoter a response to Q209L-alpha q, implying an involvement of this site in the transcriptional action of Q209L-alpha q on the prolactin promoter. The phorbol ester activator of protein kinase C, 12-O-tetradecanoylphorbol-13-acetate, stimulated (-187)PRL-CAT activity, but opposed the action of Q209L-alpha q on activity of this PRL-CAT construct. Q209L-alpha q stimulation of (-187)PRL-CAT activity was inhibited by co-expression of a dominant negative Raf mutant, Raf-C4, but not by a point mutant of Raf-C4 with reduced inhibitory properties. These results imply that activated alpha q subunits can stimulate prolactin promoter activity via a pathway that involves a Pit-1 DNA binding site(s), is opposed by protein kinase C, and is mediated by a pathway in which Raf-1 kinase plays a role.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent..., http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase, http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Prolactin, http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C, http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-raf, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Thyrotropin-Releasing..., http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate, http://linkedlifedata.com/resource/pubmed/chemical/Thyrotropin-Releasing Hormone
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0303-7207
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
112
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
249-56
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:7489829-Animals, pubmed-meshheading:7489829-Calcium-Calmodulin-Dependent Protein Kinases, pubmed-meshheading:7489829-Cell Line, pubmed-meshheading:7489829-Chloramphenicol O-Acetyltransferase, pubmed-meshheading:7489829-GTP-Binding Proteins, pubmed-meshheading:7489829-Pituitary Gland, pubmed-meshheading:7489829-Plasmids, pubmed-meshheading:7489829-Prolactin, pubmed-meshheading:7489829-Promoter Regions, Genetic, pubmed-meshheading:7489829-Protein Kinase C, pubmed-meshheading:7489829-Protein-Serine-Threonine Kinases, pubmed-meshheading:7489829-Proto-Oncogene Proteins, pubmed-meshheading:7489829-Proto-Oncogene Proteins c-raf, pubmed-meshheading:7489829-RNA, Messenger, pubmed-meshheading:7489829-Rats, pubmed-meshheading:7489829-Receptors, Thyrotropin-Releasing Hormone, pubmed-meshheading:7489829-Recombinant Fusion Proteins, pubmed-meshheading:7489829-Tetradecanoylphorbol Acetate, pubmed-meshheading:7489829-Thyrotropin-Releasing Hormone, pubmed-meshheading:7489829-Transfection
pubmed:year
1995
pubmed:articleTitle
Constitutively active Gq-alpha stimulates prolactin promoter activity via a pathway involving Raf activity.
pubmed:affiliation
Department of Physiology and Biophysics, Mount Sinai School of Medicine, City University of New York, NY 10029, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.