Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1996-1-4
|
| pubmed:abstractText |
The capacity of bone marrow-derived surface immunoglobulin-positive (sIg+) human and mouse immature B cells, generated either in vitro or in vivo, to change their light (L) chain expression, has been assayed by the number of cells which change in vitro from one type of L chain to the other type, or to no sIg at all. Immature sIg+ B cells were generated in vitro from sIg- precursor cells from human or mouse bone marrow. The immature sIg+ cells expressed RAG-1. Human sIg+ cells expressed kappa and lambda L chains in ratios between 1:1 and 3:1, whereas in mouse cells, this ratio ranged from 10:1 to 20:1. Upon reculture of the human and mouse kappa+ sIg+ cells, about half of them remained kappa+, a quarter became lambda+, and another quarter became sIg-. Between 1 and 3% expressed both kappa and lambda chains. Of the human lambda+ cells, about two-thirds remained lambda+, only 1 to 2% became kappa+, while the other third became sIg-. Again, between 1 and 3% expressed both kappa and lambda L chains. These results indicate that expression of sIgM in the B cell membrane does not terminate L chain gene rearrangement, and that some order exists in kappa versus lambda gene rearrangements. Hence, human and mouse kappa+ immature B cells can become lambda+, but very few of the lambda+ cells can become kappa+, and both can become sIg-. Further, human CD10+/sIg+ kappa+ and lambda+ cells and mouse B220low/sIglow kappa+ cells enriched from bone marrow, i.e. immature B cells differentiated in vivo, changed their Ig phenotype upon in vitro culture, but in lower frequencies. By contrast, human and mouse mature B cells did not change their L chain or Ig phenotype. Hence, at least a part of the sIg+ immature B cells in bone marrow retain the capacity to change their L chain and Ig phenotype, and this capacity is lost when they become mature, peripheral B cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0014-2980
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
25
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3108-14
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7489750-Adolescent,
pubmed-meshheading:7489750-Animals,
pubmed-meshheading:7489750-B-Lymphocytes,
pubmed-meshheading:7489750-Base Sequence,
pubmed-meshheading:7489750-Bone Marrow Cells,
pubmed-meshheading:7489750-Cell Differentiation,
pubmed-meshheading:7489750-Cells, Cultured,
pubmed-meshheading:7489750-Child,
pubmed-meshheading:7489750-Child, Preschool,
pubmed-meshheading:7489750-DNA, Complementary,
pubmed-meshheading:7489750-Gene Rearrangement, B-Lymphocyte,
pubmed-meshheading:7489750-Humans,
pubmed-meshheading:7489750-Immunoglobulin Light Chains,
pubmed-meshheading:7489750-Immunoglobulin M,
pubmed-meshheading:7489750-Infant,
pubmed-meshheading:7489750-Mice,
pubmed-meshheading:7489750-Mice, Inbred C57BL,
pubmed-meshheading:7489750-Mice, Inbred DBA,
pubmed-meshheading:7489750-Middle Aged,
pubmed-meshheading:7489750-Molecular Sequence Data,
pubmed-meshheading:7489750-RNA, Messenger
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Immature B cells from human and mouse bone marrow can change their surface light chain expression.
|
| pubmed:affiliation |
Basel Institute for Immunology, Switzerland.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|