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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1996-1-4
|
| pubmed:abstractText |
Tumour-infiltrating lymphocytes (TIL) and tumours from six patients with squamous cell carcinomas of the head and neck (SCCHN) were investigated. The six tumours all expressed major histocompatibility complex (MHC) class I antigens both in vivo and as tumor cell lines grown in vitro. In addition, the cancer cells either overexpressed the tumour-suppressor gene product p53 or harboured human papilloma virus 16/18 (HPV). The TIL were expanded in vitro in the presence of interleukin-2, immobilised anti-CD3 mAb and soluble anti-CD28 mAb. Expanded TIL cultures contained both CD4+ and CD8+ T cells, but generally contained few CD56+CD3- cells of the natural killer (NK) phenotype. CD8+ T cells dominated the individual TIL cultures from five of the six patients and showed significant autologous tumour cell lysis. In TIL cultures derived from four of these tumour-reactive TIL cultures, killing could be partially blocked by an anti-MHC class I mAb. TIL cultures reacting with autologous tumour cells also showed strong TCR/CD3-redirected cytotoxicity when assayed against hybridoma cells expressing anti-TCR/CD3 mAb as well as natural-killer(NK)-like activity. A number of TIL cultures devoid of autologous tumour cell lysis were capable of lysing the natural-killer(NK)-sensitive K562 cell line suggesting that the SCCHN cells themselves are resistant to NK-like lysis. In conclusion, TIL cultures from head and neck carcinomas contain T cells which, upon expansion in vitro, can lyse autologous tumour cells in a MHC-class-I-restricted fashion. Thus, the results of the present study document that carcinomas of the head and neck in some patients are infiltrated by cytotoxic T cell precursors potentially capable of rejecting the autologous tumour.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0340-7004
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
41
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
243-50
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:7489567-Adult,
pubmed-meshheading:7489567-Aged,
pubmed-meshheading:7489567-Aged, 80 and over,
pubmed-meshheading:7489567-Carcinoma, Squamous Cell,
pubmed-meshheading:7489567-Cytotoxicity, Immunologic,
pubmed-meshheading:7489567-Head and Neck Neoplasms,
pubmed-meshheading:7489567-Histocompatibility Antigens Class I,
pubmed-meshheading:7489567-Humans,
pubmed-meshheading:7489567-Lymphocyte Subsets,
pubmed-meshheading:7489567-Lymphocytes, Tumor-Infiltrating,
pubmed-meshheading:7489567-Middle Aged,
pubmed-meshheading:7489567-Phenotype,
pubmed-meshheading:7489567-Tumor Cells, Cultured
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Tumour-infiltrating lymphocytes mediate lysis of autologous squamous cell carcinomas of the head and neck.
|
| pubmed:affiliation |
Department of Otolaryngology, University of Copenhagen, Gentofte Hospital, Hellerup, Denmark.
|
| pubmed:publicationType |
Journal Article
|