Linked Life Data

7488947

Source:http://linkedlifedata.com/resource/pubmed/id/7488947

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1996-1-4
pubmed:abstractText
This review evaluates the applicability of the single cell gel (SCG)/comet assay as a tool for the biomonitoring of individuals accidently, environmentally or occupationally exposed to ionizing radiation. This technique detects single-strand DNA breaks, alkali-labile damage, incomplete excision repair sites and DNA:DNA crosslinking at the level of the individual cell. The advantages of this technique include: (a) data are collected at the level of the individual cell, providing information on the intercellular distribution of damage and repair; (b) only small numbers of cells are required (i.e., only a few thousand); (c) virtually any eukaryotic cell population can be used; and (d) the assay is relatively sensitive (detection limit of 5 cGy gamma rays in human lymphocytes), simple and cost effective. What the assay lacks is specificity for radiation-induced DNA damage. However, the possibility of identifying types of DNA damage specific for ionizing radiation in selected subtypes of cells may be feasible. While additional research is required before the SCG assay can readily be applied as a standard biomonitoring tool for exposure to ionizing radiation, the data collected thus far support a conclusion that such research is clearly warranted.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1066-5099
pubmed:author
pubmed:issnType
Print
pubmed:volume
13 Suppl 1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
207-14
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
The single cell gel electrophoresis/comet assay: a potential tool for detecting radiation-induced DNA damage in humans.
pubmed:affiliation
Integrated Laboratory Systems, Research Triangle Park, North Carolina 27709, USA.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, U.S. Gov't, P.H.S., Review