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rdf:type |
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lifeskim:mentions |
umls-concept:C0006675,
umls-concept:C0007600,
umls-concept:C0007634,
umls-concept:C0022022,
umls-concept:C0031586,
umls-concept:C0312738,
umls-concept:C1522424,
umls-concept:C1707719,
umls-concept:C1881379,
umls-concept:C1979572,
umls-concept:C1980044
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pubmed:issue |
2
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pubmed:dateCreated |
1995-12-21
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pubmed:abstractText |
The mouse CD5 positive pre-B cell line SPGM-1 can be induced to switch its lineage commitment towards macrophage differentiation by treatment with a combination of phorbol ester and a calcium ionophore. When cultured with these reagents the pre-B cells ceased to proliferate and rapidly became adherent to plastic surfaces. This morphological change was accompanied by the loss of pre-B cell-specific surface markers, such as PB76 and most prominently the mu heavy chain of the immunoglobulin receptor complex. In addition, the mRNA of the surrogate light chain lambda 5 disappeared while the induction of lysozyme mRNA could be detected. Differentiated SPGM-1 cells phagocytosed latex beads and showed nonspecific esterase activity. The high efficiency and speed of differentiation in this cellular system makes SPGM-1 a highly suitable model for studying the phenomenon of lineage switching during hemopoesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-3,
http://linkedlifedata.com/resource/pubmed/chemical/Ionomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Ionophores,
http://linkedlifedata.com/resource/pubmed/chemical/Muramidase,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
216
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
540-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7488145-Animals,
pubmed-meshheading:7488145-Antibodies,
pubmed-meshheading:7488145-Antigens, Surface,
pubmed-meshheading:7488145-B-Lymphocytes,
pubmed-meshheading:7488145-Blotting, Northern,
pubmed-meshheading:7488145-Cell Differentiation,
pubmed-meshheading:7488145-Cell Line,
pubmed-meshheading:7488145-Flow Cytometry,
pubmed-meshheading:7488145-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:7488145-Gene Expression,
pubmed-meshheading:7488145-Genes, fms,
pubmed-meshheading:7488145-Interleukin-3,
pubmed-meshheading:7488145-Ionomycin,
pubmed-meshheading:7488145-Ionophores,
pubmed-meshheading:7488145-Macrophages,
pubmed-meshheading:7488145-Mice,
pubmed-meshheading:7488145-Muramidase,
pubmed-meshheading:7488145-Phagocytosis,
pubmed-meshheading:7488145-Proto-Oncogenes,
pubmed-meshheading:7488145-RNA, Messenger,
pubmed-meshheading:7488145-Rats,
pubmed-meshheading:7488145-Receptor, Macrophage Colony-Stimulating Factor,
pubmed-meshheading:7488145-Tetradecanoylphorbol Acetate
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pubmed:year |
1995
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pubmed:articleTitle |
Lineage switch of a mouse pre-B cell line (SPGM-1) to macrophage-like cells after incubation with phorbol ester and calcium ionophore.
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pubmed:affiliation |
Institute of Molecular Pharmacology, Medical School Hannover, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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