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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1995-12-26
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pubmed:abstractText |
To examine the effect of disruption of the salt bridge (between Arg-164 and Asp-179 [numbering of Ambler et al. (Biochem J. 267:269-272, 1991)]) that anchors the conserved omega-loop in class A beta-lactamases, we obtained mutant enzymes with each of the 19 other amino acid residues replacing Asp-179 in the TEM beta-lactamase encoded by pUC19 and studied the level of resistance to various beta-lactams conferred by each enzyme. All mutations of Asp-179 compromised the level of resistance to ampicillin, but most of them enhanced resistance to ceftazidime. In contrast, mutations of Asp-179 generally impaired the low levels of resistance to cefepime and aztreonam. One might expect to find clinical isolates with mutant TEM beta-lactamases with replacements of Asp-179 that express an expanded spectrum of resistance to beta-lactams including ceftazidime.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7486939-1436034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7486939-1856867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7486939-1892849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7486939-1901218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7486939-1952834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7486939-2005620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7486939-2039479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7486939-2073111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7486939-2197339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7486939-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7486939-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7486939-3107125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7486939-358200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7486939-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7486939-6270337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7486939-7695296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7486939-8067751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7486939-8356032
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0066-4804
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1878-80
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7486939-Ampicillin Resistance,
pubmed-meshheading:7486939-Anti-Bacterial Agents,
pubmed-meshheading:7486939-Cephalosporin Resistance,
pubmed-meshheading:7486939-Escherichia coli,
pubmed-meshheading:7486939-Microbial Sensitivity Tests,
pubmed-meshheading:7486939-Mutation,
pubmed-meshheading:7486939-Staphylococcus aureus,
pubmed-meshheading:7486939-Sulfur Radioisotopes,
pubmed-meshheading:7486939-beta-Lactam Resistance,
pubmed-meshheading:7486939-beta-Lactamases,
pubmed-meshheading:7486939-beta-Lactams
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pubmed:year |
1995
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pubmed:articleTitle |
Effects of Asp-179 mutations in TEMpUC19 beta-lactamase on susceptibility to beta-lactams.
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pubmed:affiliation |
Department of Medicine, Wayne State University, Detroit, Michigan 48202, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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