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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5238
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pubmed:dateCreated |
1995-12-13
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pubmed:abstractText |
Polychlorinated biphenyls (PCBs) typify a class of stable aromatic pollutants that are targeted by bioremediation strategies. In the aerobic degradation of biphenyl by bacteria, the key step of ring cleavage is catalyzed by an Fe(II)-dependent extradiol dioxygenase. The crystal structure of 2,3-dihydroxybiphenyl 1,2-dioxygenase from a PCB-degrading strain of Pseudomonas cepacia has been determined at 1.9 angstrom resolution. The monomer comprises amino- and carboxyl-terminal domains. Structural homology between and within the domains reveals evolutionary relationships within the extradiol dioxygenase family. The iron atom has five ligands in square pyramidal geometry: one glutamate and two histidine side chains, and two water molecules.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
270
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
976-80
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7481800-Amino Acid Sequence,
pubmed-meshheading:7481800-Binding Sites,
pubmed-meshheading:7481800-Biodegradation, Environmental,
pubmed-meshheading:7481800-Crystallography, X-Ray,
pubmed-meshheading:7481800-Dioxygenases,
pubmed-meshheading:7481800-Evolution, Molecular,
pubmed-meshheading:7481800-Ferrous Compounds,
pubmed-meshheading:7481800-Hydrogen Bonding,
pubmed-meshheading:7481800-Ligands,
pubmed-meshheading:7481800-Models, Molecular,
pubmed-meshheading:7481800-Molecular Sequence Data,
pubmed-meshheading:7481800-Oxygen,
pubmed-meshheading:7481800-Oxygenases,
pubmed-meshheading:7481800-Polychlorinated Biphenyls,
pubmed-meshheading:7481800-Protein Conformation,
pubmed-meshheading:7481800-Protein Structure, Secondary,
pubmed-meshheading:7481800-Pseudomonas,
pubmed-meshheading:7481800-Sequence Alignment
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pubmed:year |
1995
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pubmed:articleTitle |
Crystal structure of the biphenyl-cleaving extradiol dioxygenase from a PCB-degrading pseudomonad.
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pubmed:affiliation |
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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