Linked Life Data

747

Source:http://linkedlifedata.com/resource/pubmed/id/747

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1976-2-19
pubmed:abstractText
A simple and convenient colorimetric method is described for the quantitative determination of 5-aminosalicylic acid (5-ASA) and N-acetyl-5-ASA in urine and feces after oral administration of salicylazosulfa-pyridine (SASP), the drug of choice in the treatment of ulcerative colitis. N-acetyl-5-ASA is extracted directly from the acidified biological specimen, deacetylated, and the liberated 5-ASA subjected to a modified Bratton-Marshall reaction. The 5-ASA present in the specimen must be acetylated with acetic anhydride prior to extraction. The violet colored product of the Bratton-Marshall reaction has a lambdamax of 560 nm and conforms to Beer's law over the concentration range of 0-70 umg/ml. Average recoveries (+/- S.D., N = 6) OF 5-ASA added to rat and human urine and rat fecal homogenates were 91.6 +/- 4.9%, 102 +/- 6.0%, and 71.0 +/- 4.8%, respectively. Interference by SASP and its sulfapyridine metabolities is negligible. As demonstrated, the colorimetric method is of sufficient sensitivity for application in most metabolic and pharmacokinetic studies conducted with SASP in laboratory animals and man.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0034-5164
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
571-81
pubmed:dateRevised
2009-10-27
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
Colorimetric determination of 5-aminosalicylic acid and its N-acetylated metabolite on urine and feces.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.