Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
1995-11-30
pubmed:abstractText
Although the incidence of Gram-positive sepsis has risen strongly, it is unclear how Gram-positive organisms (without endotoxin) initiate septic shock. We investigated whether two cell wall components from Staphylococcus aureus, peptidoglycan (PepG) and lipoteichoic acid (LTA), can induce the inflammatory response and multiple organ dysfunction syndrome (MODS) associated with septic shock caused by Gram-positive organisms. In cultured macrophages, LTA (10 micrograms/ml), but not PepG (100 micrograms/ml), induces the release of nitric oxide measured as nitrite. PepG, however, caused a 4-fold increase in the production of nitrite elicited by LTA. Furthermore, PepG antibodies inhibited the release of nitrite elicited by killed S. aureus. Administration of both PepG (10 mg/kg; i.v.) and LTA (3 mg/kg; i.v.) in anesthetized rats resulted in the release of tumor necrosis factor alpha and interferon gamma and MODS, as indicated by a decrease in arterial oxygen pressure (lung) and an increase in plasma concentrations of bilirubin and alanine aminotransferase (liver), creatinine and urea (kidney), lipase (pancreas), and creatine kinase (heart or skeletal muscle). There was also the expression of inducible nitric oxide synthase in these organs, circulatory failure, and 50% mortality. These effects were not observed after administration of PepG or LTA alone. Even a high dose of LTA (10 mg/kg) causes only circulatory failure but no MODS. Thus, our results demonstrate that the two bacterial wall components, PepG and LTA, work together to cause systemic inflammation and multiple systems failure associated with Gram-positive organisms.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-1714727, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-1899557, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-1953116, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-1984950, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-2037372, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-2040686, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-2206394, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-2221111, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-2244897, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-2642920, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-2664516, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-6589747, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-6589748, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-7509750, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-7510950, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-7516310, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-7527832, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-7542534, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-7739024, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-7799056, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-7806384, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-7852050, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-7890367, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-7996048, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-8026188, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-8138241, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-8215727, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-8267486, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-8277249, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-8379598, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-8406805, http://linkedlifedata.com/resource/pubmed/commentcorrection/7479784-8479467
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
92
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10359-63
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:7479784-Animals, pubmed-meshheading:7479784-Cell Line, pubmed-meshheading:7479784-Cell Wall, pubmed-meshheading:7479784-Enzyme Induction, pubmed-meshheading:7479784-Hemodynamics, pubmed-meshheading:7479784-Interferon-gamma, pubmed-meshheading:7479784-Isoenzymes, pubmed-meshheading:7479784-Lipopolysaccharides, pubmed-meshheading:7479784-Lung, pubmed-meshheading:7479784-Macrophages, pubmed-meshheading:7479784-Male, pubmed-meshheading:7479784-Mice, pubmed-meshheading:7479784-Multiple Organ Failure, pubmed-meshheading:7479784-Nitric Oxide Synthase, pubmed-meshheading:7479784-Organ Specificity, pubmed-meshheading:7479784-Peptidoglycan, pubmed-meshheading:7479784-Rats, pubmed-meshheading:7479784-Rats, Wistar, pubmed-meshheading:7479784-Shock, Septic, pubmed-meshheading:7479784-Staphylococcal Infections, pubmed-meshheading:7479784-Staphylococcus aureus, pubmed-meshheading:7479784-Teichoic Acids, pubmed-meshheading:7479784-Tumor Necrosis Factor-alpha, pubmed-meshheading:7479784-Virulence
pubmed:year
1995
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