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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
1995-11-30
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pubmed:abstractText |
We have previously reported an enhanced version of sequencing by hybridization (SBH), termed positional SBH (PSBH). PSBH uses partially duplex probes containing single-stranded 3' overhangs, instead of simple single-stranded probes. Stacking interactions between the duplex probe and a single-stranded target allow us to reduce the probe sizes required to 5-base single-stranded overhangs. Here we demonstrate the use of PSBH to capture relatively long single-stranded DNA targets and perform standard solid-state Sanger sequencing on these primer-template complexes without ligation. Our results indicate that only 5 bases of known terminal sequence are required for priming. In addition, the partially duplex probes have the ability to capture their specific target from a mixture of five single-stranded targets with different 3'-terminal sequences. This indicates the potential utility of the PSBH approach to sequence mixtures of DNA targets without prior purification.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479746-1465615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479746-1769648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479746-1946427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479746-2265749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479746-2379835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479746-2668874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479746-2737674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479746-2780548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479746-3010776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479746-3256722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479746-6259625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479746-6269069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479746-7937073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479746-8159709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479746-8197176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479746-8483939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479746-8503011
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
92
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10162-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1995
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pubmed:articleTitle |
A DNA sequencing strategy that requires only five bases of known terminal sequence for priming.
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pubmed:affiliation |
Sequenom, Inc., Boston, MA 02210, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.
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