|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
20
|
|
pubmed:dateCreated |
1995-12-21
|
|
pubmed:abstractText |
We present a method for the isolation of YAC insert sequences by representational difference analysis (RDA). To achieve maximal representation of the sequences, the amplicons were generated from a Mbol digestion product. RDA was performed using a 970 kb insert YAC clone. After two rounds of re-association and selective amplification 92% of the difference product represented sequences derived from the YAC insert. Twenty insert-specific sequence-tagged sites were readily defined. The difference product was also successfully used to isolate microsatellite markers, to identify clones from a human PAC library and as a chromosome painting probe in fluorescence in situ hybridization.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479076-1639380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479076-2138783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479076-2235490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479076-2294591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479076-2408039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479076-2734098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479076-2973607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479076-2991893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479076-3476958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479076-6310992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479076-6326095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479076-6722869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479076-6989766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479076-7597059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479076-7774906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479076-7959755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479076-8136839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479076-8259213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7479076-8438152
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0305-1048
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
25
|
|
pubmed:volume |
23
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
4127-33
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:7479076-Base Sequence,
pubmed-meshheading:7479076-Carcinoma,
pubmed-meshheading:7479076-Chromosomes, Artificial, Yeast,
pubmed-meshheading:7479076-Chromosomes, Human, Pair 13,
pubmed-meshheading:7479076-Cloning, Molecular,
pubmed-meshheading:7479076-DNA,
pubmed-meshheading:7479076-Dinucleotide Repeats,
pubmed-meshheading:7479076-Humans,
pubmed-meshheading:7479076-In Situ Hybridization, Fluorescence,
pubmed-meshheading:7479076-Molecular Sequence Data,
pubmed-meshheading:7479076-Pancreatic Neoplasms,
pubmed-meshheading:7479076-Polymerase Chain Reaction,
pubmed-meshheading:7479076-Sequence Tagged Sites,
pubmed-meshheading:7479076-Translocation, Genetic,
pubmed-meshheading:7479076-Tumor Cells, Cultured
|
|
pubmed:year |
1995
|
|
pubmed:articleTitle |
Isolation of YAC insert sequences by representational difference analysis.
|
|
pubmed:affiliation |
Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21205-2196, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|
