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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1995-12-26
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pubmed:abstractText |
Previous experiments have demonstrated that the regulation of E2F-1 transcription factor activity is critical for the maintenance of normal cell proliferation control. Regulation of E2F-1 is accomplished through at least two mechanisms: posttranslational regulation by binding proteins such as Rb and transcriptional regulation of the E2F-1 gene. The E2F-1 gene promoter has recently been isolated to examine this latter aspect of E2F-1 regulation. Preliminary studies demonstrate that the E2F-1 promoter is under E2F-dependent negative control during the cell growth response, being transcriptionally repressed through E2F sites in G0 and early G1. We now demonstrate that the presence of an E2F DNA-binding complex containing the Rb-related p130 protein (Rb2) correlates with E2F-1 gene repression and that overexpression of p130 inhibits transcription from the E2F-1 promoter. Moreover, D-type cyclin-dependent kinase activity specifically activates the E2F-1 promoter by relieving E2F-mediated repression but is inhibited by coexpression of the cdk4 and cdk6 inhibitor p16 (CDKN2, MTS1, INK4). Taken together, these findings suggest that E2F-1 gene expression is controlled during cell cycle progression by a regulatory network involving at least one oncogene (cyclin D1) and several potential tumor suppressor genes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin D1,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase 4,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/E2F Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/E2F1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Retinoblastoma-Binding Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/Retinoblastoma-Like Protein p130,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor DP1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0950-9232
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1685-92
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7478595-Animals,
pubmed-meshheading:7478595-Base Sequence,
pubmed-meshheading:7478595-Carrier Proteins,
pubmed-meshheading:7478595-Cell Cycle,
pubmed-meshheading:7478595-Cell Cycle Proteins,
pubmed-meshheading:7478595-Cell Division,
pubmed-meshheading:7478595-Cell Line,
pubmed-meshheading:7478595-Cyclin D1,
pubmed-meshheading:7478595-Cyclin-Dependent Kinase 4,
pubmed-meshheading:7478595-Cyclin-Dependent Kinases,
pubmed-meshheading:7478595-Cyclins,
pubmed-meshheading:7478595-DNA-Binding Proteins,
pubmed-meshheading:7478595-E2F Transcription Factors,
pubmed-meshheading:7478595-E2F1 Transcription Factor,
pubmed-meshheading:7478595-Gene Expression Regulation,
pubmed-meshheading:7478595-Molecular Sequence Data,
pubmed-meshheading:7478595-Oligodeoxyribonucleotides,
pubmed-meshheading:7478595-Oncogene Proteins,
pubmed-meshheading:7478595-Phosphoproteins,
pubmed-meshheading:7478595-Promoter Regions, Genetic,
pubmed-meshheading:7478595-Protein Processing, Post-Translational,
pubmed-meshheading:7478595-Proteins,
pubmed-meshheading:7478595-Proto-Oncogene Proteins,
pubmed-meshheading:7478595-Recombinant Proteins,
pubmed-meshheading:7478595-Retinoblastoma-Binding Protein 1,
pubmed-meshheading:7478595-Retinoblastoma-Like Protein p130,
pubmed-meshheading:7478595-Transcription, Genetic,
pubmed-meshheading:7478595-Transcription Factor DP1,
pubmed-meshheading:7478595-Transcription Factors,
pubmed-meshheading:7478595-Transfection
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pubmed:year |
1995
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pubmed:articleTitle |
Regulation of E2F-1 gene expression by p130 (Rb2) and D-type cyclin kinase activity.
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pubmed:affiliation |
Department of Carcinogenesis, University of Texas M.D. Anderson Cancer Center, Smithville 78957, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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