|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
7
|
|
pubmed:dateCreated |
1995-11-28
|
|
pubmed:abstractText |
FDC-P1 haemopoietic cells were used to select mutations of c-fms that constitutively activate the receptor for macrophage-colony stimulating factor (M-CSF or CSF-1). One mutation changed Ser 929 to Gly within a Ser/Gly rich region of the C-terminal tail and a second changed a nearby, highly conserved Leu 926 for Pro. A third mutation (D802V) changed Asp 802 to Val within the alpha L12/beta 9 region of the tyrosine kinase domain, so supporting the crystallographic evidence that this region triggers kinase activation. A c-kit mutation exactly equivalent to D802V was previously identified in a leukamic cell line and was demonstrated here to be transforming. Surprisingly, although D802V potently transformed FDC-P1 cells, it could not induce Rat-2 fibroblast foci, even in the presence of M-CSF. It is suggested that the accelerated receptor degradation induced by D802V may account for its cell specific effect.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Colony-Stimulating Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-kit,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Stem Cell Factor
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0950-9232
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
5
|
|
pubmed:volume |
11
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1347-56
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:7478556-Amino Acid Sequence,
pubmed-meshheading:7478556-Animals,
pubmed-meshheading:7478556-Base Sequence,
pubmed-meshheading:7478556-Cell Division,
pubmed-meshheading:7478556-Cell Transformation, Neoplastic,
pubmed-meshheading:7478556-DNA, Complementary,
pubmed-meshheading:7478556-Hematopoietic Stem Cells,
pubmed-meshheading:7478556-Humans,
pubmed-meshheading:7478556-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:7478556-Mice,
pubmed-meshheading:7478556-Molecular Sequence Data,
pubmed-meshheading:7478556-Mutation,
pubmed-meshheading:7478556-Oligodeoxyribonucleotides,
pubmed-meshheading:7478556-Phosphorylation,
pubmed-meshheading:7478556-Protein-Tyrosine Kinases,
pubmed-meshheading:7478556-Proto-Oncogene Proteins c-kit,
pubmed-meshheading:7478556-Receptor, Macrophage Colony-Stimulating Factor,
pubmed-meshheading:7478556-Receptors, Cell Surface,
pubmed-meshheading:7478556-Sequence Alignment,
pubmed-meshheading:7478556-Stem Cell Factor
|
|
pubmed:year |
1995
|
|
pubmed:articleTitle |
Selection of activating mutations of c-fms in FDC-P1 cells.
|
|
pubmed:affiliation |
Department of Chemical Pathology, Royal Postgraduate Medical School, Hammersmith Hospital, London.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
