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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1995-12-14
|
| pubmed:abstractText |
We previously reported evidence of beta-adrenoceptor-mediated induction of IL-1 beta mRNA in the rat hypothalamus. The present in vitro studies using northern blot analysis showed that the beta-adrenoceptor agonist isoproterenol (1 x 10(-8) to 1 x 10(-5) M) caused a marked induction of IL-1 beta mRNA in microglia, but not in astrocytes. This induction was remarkably suppressed by pretreatment of cells with the beta-adrenoceptor antagonist propranolol. These phenomena were confirmed by in situ hybridization with digoxigenin-labelled IL-1 beta RNA probe. Furthermore, dibutyryl cyclicAMP (dbcAMP) (5 x 10(-4) and 5 x 10(-5) M) markedly induced IL-1 beta mRNA in microglia. The intracellular level of cAMP in microglia was elevated in a dose-dependent manner when they were treated with isoproterenol, and this elevation was completely blocked by propranolol. The induction of IL-1 beta mRNA by either isoproterenol or dbcAMP was strongly inhibited by a cAMP-dependent protein kinase inhibitor, H8. These results, taken together, suggest that (1) microglia primarily induce IL-1 beta mRNA by stimulation of beta-adrenoceptors, and (2) cAMP and cAMP-dependent protein kinase presumably participate in a signal transduction mechanism involved in the induction of IL-1 beta mRNA via beta-adrenoceptors.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic beta-Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP-Dependent Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Isoproterenol,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenergic, beta
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0168-0102
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
22
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
399-409
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7478305-Adrenergic beta-Agonists,
pubmed-meshheading:7478305-Animals,
pubmed-meshheading:7478305-Astrocytes,
pubmed-meshheading:7478305-Blotting, Northern,
pubmed-meshheading:7478305-Cells, Cultured,
pubmed-meshheading:7478305-Cyclic AMP,
pubmed-meshheading:7478305-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:7478305-Immunohistochemistry,
pubmed-meshheading:7478305-In Situ Hybridization,
pubmed-meshheading:7478305-Interleukin-1,
pubmed-meshheading:7478305-Isoproterenol,
pubmed-meshheading:7478305-Microglia,
pubmed-meshheading:7478305-RNA, Messenger,
pubmed-meshheading:7478305-RNA Probes,
pubmed-meshheading:7478305-Rats,
pubmed-meshheading:7478305-Rats, Sprague-Dawley,
pubmed-meshheading:7478305-Receptors, Adrenergic, beta,
pubmed-meshheading:7478305-Signal Transduction
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Participation of cAMP and cAMP-dependent protein kinase in beta-adrenoceptor-mediated interleukin-1 beta mRNA induction in cultured microglia.
|
| pubmed:affiliation |
Department of Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|