Linked Life Data

7476946

Source:http://linkedlifedata.com/resource/pubmed/id/7476946

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1995-12-8
pubmed:abstractText
Gene recA from Pseudomonas aeruginosa was cloned into pUC19 vector under lacZ promoter. The expressed protein appeared to be modified, the aminoterminal part of deduced amino acid sequence of the RecAPa protein was found elongated by a polypeptide of 10 amino acids. The modified protein named RecA*Pa completely replaces RecAEc from E. coli in vivo recombination. In vitro RecA*Pa promotes the homologous strand transfer from a short linear duplex DNA fragment (346 bp) into circular single-stranded DNA (8196 n) being 5 times more active than RecAEc. However, when the length of dsDNA increased the difference between two proteins becomes negligible. To understand the reasons, some properties of RecA*Pa and RecAEc were compared. The former was shown to be more active both in binding to ssDNA in ssDNA-dependent ATP hydrolysis. The Rec*Pa protein showed also a high affinity to dsDNA, even at a physiological pH which is known to be unfavorable for RecAEc/dsDNA binding. However, both proteins equally catalyzed the dsDNA-dependent ATP hydrolysis; we suggest that this is crucial for a full-length DNA strand transfer recombination reaction.
pubmed:language
rus
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0026-8984
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
801-12
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:articleTitle
[Recombination properties of the modified Pseudomonas aeruginosa RecA protein].
pubmed:publicationType
Journal Article, English Abstract