Linked Life Data

7476918

Source:http://linkedlifedata.com/resource/pubmed/id/7476918

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1995-12-20
pubmed:databankReference
pubmed:abstractText
Five distinct cDNA clones encoding four different isoforms of human prostaglandin (PG) E receptor EP3 subtype were isolated from a human kidney cDNA library. Two cDNA clones differed only in their 3'-untranslated regions. The four isoforms, tentatively named EP3-I, EP3-II, EP3-III, and EP3-IV, which were generated by alternative mRNA splicing, had identical amino acid sequences except for their different carboxyl-terminal tails. Transfection experiments revealed that all the four isoforms show high binding affinities to PGE2, PGE1, and M&B28767, an EP3-specific agonist, whereas their downstream signaling pathways are divergent. M&B28767 increased cAMP concentrations in cells expressing EP3-II and EP3-IV, whereas it inhibited forskolin-induced cAMP accumulations in cells expressing all EP3 isoforms. M&B28767 also stimulated phosphoinositide turnover in cells expressing EP3-I and EP3-II. Northern blot analysis revealed that the EP3 gene is expressed in a wide variety of human tissues. The human EP3 mRNA was present most abundantly in the kidney, pancreas, and uterus. A substantial expression was also detected in the heart, liver, skeletal muscle, small intestine, colon, prostate, ovary, and testis. Furthermore, reverse transcription-polymerase chain reaction analysis demonstrated tissue-specific expressions of the five different EP3 mRNA species. The present study suggests the presence of the multiple systems of PGE2/EP3 isoforms and leads to the better understanding of its physiological and pathophysiological implications in humans.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0026-895X
pubmed:author
pubmed:issnType
Print
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
869-79
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7476918-Alternative Splicing, pubmed-meshheading:7476918-Amino Acid Sequence, pubmed-meshheading:7476918-Animals, pubmed-meshheading:7476918-Base Sequence, pubmed-meshheading:7476918-Blotting, Northern, pubmed-meshheading:7476918-Blotting, Southern, pubmed-meshheading:7476918-CHO Cells, pubmed-meshheading:7476918-Cloning, Molecular, pubmed-meshheading:7476918-Cricetinae, pubmed-meshheading:7476918-Cyclic AMP, pubmed-meshheading:7476918-Dinoprostone, pubmed-meshheading:7476918-Humans, pubmed-meshheading:7476918-Inositol 1,4,5-Trisphosphate, pubmed-meshheading:7476918-Molecular Sequence Data, pubmed-meshheading:7476918-Organ Specificity, pubmed-meshheading:7476918-Polymerase Chain Reaction, pubmed-meshheading:7476918-Receptors, Prostaglandin E, pubmed-meshheading:7476918-Second Messenger Systems
pubmed:year
1995
pubmed:articleTitle
Molecular cloning and expression of multiple isoforms of human prostaglandin E receptor EP3 subtype generated by alternative messenger RNA splicing: multiple second messenger systems and tissue-specific distributions.
pubmed:affiliation
Department of Medicine, Faculty of Medicine, Kyoto University, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't