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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1995-12-1
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pubmed:abstractText |
Individuals infected with the human immunodeficiency virus (HIV) experience a marked loss of CD4+ T lymphocytes, leading to fatal immunodeficiency. The mechanisms causing the depletion of these cells are not yet understood. In this study, we observed that CD4+ T lymphocytes from HIV type 1 (HIV-1)-infected and uninfected individuals rapidly lysed B lymphoblasts expressing the HIV-1 envelope glycoprotein on the cell surface and Jurkat cells expressing the complete virus. Contact of uninfected CD4+ T cells with envelope glycoprotein-expressing cells also resulted in the lysis of the uninfected CD4+ T cells. Cytolysis did not require priming or in vitro stimulation of the CD4+ T cells and was not restricted by major histocompatibility complex molecules. Cytotoxicity was inhibited by soluble CD4 and anti-CD4 monoclonal antibodies that block binding of CD4 to gp120. In addition, neutralizing anti-CD4 and anti-gp120 monoclonal antibodies which block postbinding membrane fusion events and syncytium formation also inhibited cell lysis, suggesting that identical mechanisms in HIV-infected cultures underlie cell-cell fusion and the cytolysis observed. However, cytotoxicity was not always accompanied by the formation of visible syncytia. Rapid cell lysis after contact of uninfected and HIV-1-infected CD4+ T cells may explain CD4+ T-cell depletion in the absence of detectable syncytia in infected individuals. Moreover, because of its vigor, lysis of envelope-expressing targets by contact with unprimed CD4+ T lymphocytes may at first glance resemble antigen-specific immune responses and should be excluded when cytotoxic T-lymphocyte responses in infected individuals and vaccinees are evaluated.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-1319711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-1346269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-1349322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-1360665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-1383338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-1402655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-14278051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-1637373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-1672248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-1683728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-1698911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-1742075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-1744586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-1948066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-1985202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-2106442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-2147200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-2451288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-2460873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-2463308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-2480604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-2522999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-2536094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-2536767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-2950525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-2957508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-2966682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-3010463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-3016552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-3047430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-3127528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-3156183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-6200935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-6288803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-6327821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-7678973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-7689848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-7824947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-7888228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-7915115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7474110-8093551
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
69
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6925-31
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7474110-Antibodies, Blocking,
pubmed-meshheading:7474110-Antibodies, Monoclonal,
pubmed-meshheading:7474110-Antigens, CD,
pubmed-meshheading:7474110-Antigens, CD4,
pubmed-meshheading:7474110-CD4-Positive T-Lymphocytes,
pubmed-meshheading:7474110-CD8-Positive T-Lymphocytes,
pubmed-meshheading:7474110-Cell Communication,
pubmed-meshheading:7474110-Cell Line,
pubmed-meshheading:7474110-Cytotoxicity, Immunologic,
pubmed-meshheading:7474110-Giant Cells,
pubmed-meshheading:7474110-HIV Envelope Protein gp120,
pubmed-meshheading:7474110-HIV Infections,
pubmed-meshheading:7474110-HIV-1,
pubmed-meshheading:7474110-Humans,
pubmed-meshheading:7474110-Immunophenotyping,
pubmed-meshheading:7474110-Major Histocompatibility Complex,
pubmed-meshheading:7474110-Reference Values,
pubmed-meshheading:7474110-T-Lymphocytes
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pubmed:year |
1995
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pubmed:articleTitle |
Contact of human immunodeficiency virus type 1-infected and uninfected CD4+ T lymphocytes is highly cytolytic for both cells.
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pubmed:affiliation |
Institute for Virology and Immunobiology, Würzburg University, Germany.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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