Linked Life Data

7473135

Source:http://linkedlifedata.com/resource/pubmed/id/7473135

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-12-19
pubmed:abstractText
Anandamide (arachidonylethanolamide), isolated from the porcine brain, and 2-arachidonyl-glycerol (2-Ara-Gl), derived from the canine gut, are two recently identified putative endogenous cannabinoid receptor ligands. Both ligands have been reported to possess binding affinity for cannabinoid receptor subtypes, CB1 and CB2. The objective of the present studies was to investigate the immunomodulatory effects of both of these ligands in B6C3F1 mouse splenocytes. 2-Ara-Gl produced a marked and dose-related inhibition of the mixed lymphocyte response, anti-CD3 mAb-induced T-cell proliferation and LPS-induced B-cell proliferation, whereas having no inhibitory effect on phorbol-12-myristate-13-acetate/ionomycin-induced cell proliferation. Interestingly, the inhibitory effects by 2-Ara-Gl on proliferation were at least dependent in part on cell density. At high cell density, 2-Ara-Gl enhanced lymphoproliferation whereas exhibiting marked inhibitory activity at low cell density. Similarly, in vitro primary immunoglobulin M antibody-forming cell responses which are dependent on high cell density also were found to be enhanced by 2-Ara-Gl. Conversely, anandamide exhibited no inhibitory effects on cell proliferative responses to stimulation by anti-CD3 mAb, lipopolysaccharide or phorbol-12-myristate-13-acetate/ionomycin treatment. Anandamide also showed no effect on the in vitro sheep erythrocyte antibody-forming cell response. Although shown previously to markedly inhibit forskolin-stimulated cyclic AMP accumulation, 2-Ara-Gl exhibited no effect on basal adenylate cyclase activity in splenocytes. Additionally, anandamide showed negligible inhibitory effects at extremely high concentrations on forskolin-stimulated adenylate cyclase activity and no effect on basal adenylate cyclase activity in splenocytes.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-3565
pubmed:author
pubmed:issnType
Print
pubmed:volume
275
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
529-36
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:7473135-Adenylate Cyclase, pubmed-meshheading:7473135-Adjuvants, Immunologic, pubmed-meshheading:7473135-Animals, pubmed-meshheading:7473135-Antibody Formation, pubmed-meshheading:7473135-Antibody-Producing Cells, pubmed-meshheading:7473135-Arachidonic Acids, pubmed-meshheading:7473135-Cells, Cultured, pubmed-meshheading:7473135-Enzyme Activation, pubmed-meshheading:7473135-Female, pubmed-meshheading:7473135-Forskolin, pubmed-meshheading:7473135-Glycerides, pubmed-meshheading:7473135-Ligands, pubmed-meshheading:7473135-Lymphocyte Activation, pubmed-meshheading:7473135-Mice, pubmed-meshheading:7473135-Polyunsaturated Alkamides, pubmed-meshheading:7473135-Receptors, Cannabinoid, pubmed-meshheading:7473135-Receptors, Drug, pubmed-meshheading:7473135-Spleen, pubmed-meshheading:7473135-T-Lymphocytes
pubmed:year
1995
pubmed:articleTitle
Effects of putative cannabinoid receptor ligands, anandamide and 2-arachidonyl-glycerol, on immune function in B6C3F1 mouse splenocytes.
pubmed:affiliation
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't