Linked Life Data

7472653

Source:http://linkedlifedata.com/resource/pubmed/id/7472653

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1995-12-19
pubmed:abstractText
In a previous study we demonstrated that 13-hydroxyoctadecadienoic acid (13-HODE), a 15-lipoxygenase metabolite of linoleic acid is incorporated into epidermal phosphatidyl 4,5-bisphosphate (PtdIns 4,5-P2) and released as 13-HODE-containing-diacylglycerol (13-HODE-DAG). In vitro, 13-HODE-DAG was shown to selectively inhibit epidermal total protein kinase C (PKC-beta) activity. To determine whether these observations are relevant in vivo, guinea pigs were made essential fatty acid deficient (EFAD) by feeding them a basal diet supplemented with 4% hydrogenated coconut oil for 8 wk. Tissue levels of putative 13-HODE-DAG, protein kinase C (PKC) isozymes and tissue hyperproliferation were determined in the epidermal preparations from skin of control safflower oil-fed guinea pigs, those fed EFAD diet and those fed EFAD diet followed by the control diet for 2 wk. Our data revealed that cutaneous 13-HODE and 13-HODE-DAG were significantly lower in EFAD animals than in safflower-fed controls. These reductions were associated with both elevated epidermal hyperproliferation and elevated expressions and activities of PKC-alpha and beta-isozymes. Refeeding the animals with safflower oil for 2 wk replenished tissue levels of 13-HODE-DAG, which inversely correlated with the selective down regulation of PKC-beta expression and activity and the reversal of hyperproliferation. In contrast, although, the expression and activity of PKC-alpha was elevated in the epidermis of the EFAD guinea pigs, this elevated PKC-alpha expression was not down regulated after refeeding the safflower oil diet to the animals.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-3166
pubmed:author
pubmed:issnType
Print
pubmed:volume
125
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2741-50
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:7472653-Animal Nutritional Physiological Phenomena, pubmed-meshheading:7472653-Animals, pubmed-meshheading:7472653-Blotting, Western, pubmed-meshheading:7472653-Cell Division, pubmed-meshheading:7472653-Cocos, pubmed-meshheading:7472653-Diet, pubmed-meshheading:7472653-Dietary Fats, pubmed-meshheading:7472653-Diglycerides, pubmed-meshheading:7472653-Down-Regulation, pubmed-meshheading:7472653-Fatty Acids, Essential, pubmed-meshheading:7472653-Guinea Pigs, pubmed-meshheading:7472653-Isoenzymes, pubmed-meshheading:7472653-Linoleic Acid, pubmed-meshheading:7472653-Linoleic Acids, pubmed-meshheading:7472653-Male, pubmed-meshheading:7472653-Plant Oils, pubmed-meshheading:7472653-Protein Kinase C, pubmed-meshheading:7472653-Safflower Oil, pubmed-meshheading:7472653-Signal Transduction, pubmed-meshheading:7472653-Skin
pubmed:year
1995
pubmed:articleTitle
Nutritional modulation of guinea pig skin hyperproliferation by essential fatty acid deficiency is associated with selective down regulation of protein kinase C-beta.
pubmed:affiliation
Department of Dermatology, University of California, Davis 95616, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.