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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1995-12-26
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pubmed:abstractText |
We have developed the coexpression system of both delta-opioid receptor (DOR1) and M2-muscarinic receptor (M2) which mediate agonist-evoked currents due to common post-receptor mechanisms including Gi1 and phospholipase C (PLC) activation in Xenopus oocytes reconstituted with Gi1 alpha. The DOR1-currents by 100 nM D-Ser2-leu-enkephalin-Thr6 (DSLET) were selectively desensitized by 10 nM phorbol 12-myristate 13-acetate (PMA). The PMA-desensitization of DSLET-currents was abolished in the presence of calphostin C, a protein kinase C inhibitor, or reversed by an intracellular injection of calcineurin, a protein phosphatase 2B. When a higher concentration (3 microM) of DSLET was used, DSLET-currents were rapidly desensitized by repeated challenges of DSLET itself. However, repeated challenges of 10 microM ACh caused no influence on such DSLET- or M2-currents. The desensitization of DSLET-currents was selectively reversed by protein kinase C inhibitors. Similar results were also obtained with various delta-opioid agonists. These results suggest that protein kinase C is involved in the homologous desensitization of delta-opioid receptors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Enkephalin, Leucine,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Muscarinic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid, delta,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases,
http://linkedlifedata.com/resource/pubmed/chemical/enkephalin, Ser(2), Leu(5), Thr(6)-
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0270-6474
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7485-99
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pubmed:dateRevised |
2008-8-22
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pubmed:meshHeading |
pubmed-meshheading:7472500-Animals,
pubmed-meshheading:7472500-Electrophysiology,
pubmed-meshheading:7472500-Enkephalin, Leucine,
pubmed-meshheading:7472500-Enzyme Activation,
pubmed-meshheading:7472500-GTP-Binding Proteins,
pubmed-meshheading:7472500-Injections,
pubmed-meshheading:7472500-Oocytes,
pubmed-meshheading:7472500-Protein Kinase C,
pubmed-meshheading:7472500-RNA,
pubmed-meshheading:7472500-Receptors, Muscarinic,
pubmed-meshheading:7472500-Receptors, Opioid, delta,
pubmed-meshheading:7472500-Signal Transduction,
pubmed-meshheading:7472500-Tetradecanoylphorbol Acetate,
pubmed-meshheading:7472500-Type C Phospholipases,
pubmed-meshheading:7472500-Xenopus laevis
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pubmed:year |
1995
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pubmed:articleTitle |
Protein kinase C involvement in homologous desensitization of delta-opioid receptor coupled to Gi1-phospholipase C activation in Xenopus oocytes.
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pubmed:affiliation |
Department of Pharmacology, Yokohama City University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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