Linked Life Data

7462696

Source:http://linkedlifedata.com/resource/pubmed/id/7462696

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1981-4-24
pubmed:abstractText
A cold-recombinant virus CR 22, was derived from an attenuated cold-adapted parent strain. A/Ann Arbor/6/60 (H2N2), and a wild-type parent strain, A/Victoria/3/75 (H3N2). Antigenic analysis showed that CR 22 possesses the hemagglutinin and neruaminidase surface antigens derived from the A/Victoria/3/75 (H3N2) parent. From studies of virus-induced polypeptides using polyacrylamide gel electrophoresis, it was deduced that a polymerase protein, P1, is coded and by an RNA segment derived from the wild-type parent; all other genetic elements are derived from the attenuated parent. The attenuated parent and the recombinant CR 22 both possess temperature-sensitive (ts) lesions, evident by restriction of multiplication in fertile chicken eggs or in Madin-Darby canine kidney cell cultures at greater than or equal to 38 C. Genetic analysis of CR 22 by complementation tests using Hong Kong and Wilson Smith neurotropic ts mutants gave evidence for a ts lesion in the genetic elements coding for complementary RNA.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-1899
pubmed:author
pubmed:issnType
Print
pubmed:volume
142
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
850-6
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Studies with a cold-recombinant A/Victoria/3/75 (H3N2) virus. I. biologic, genetic, and biochemical characterization.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.