Linked Life Data

7460912

Source:http://linkedlifedata.com/resource/pubmed/id/7460912

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1981-4-13
pubmed:abstractText
Basement membranes were isolated from rabbit kidney using the detergent N-dodecyl sarcosine. Treatment of the membranes with 6 M urea or 5% sodium dodecyl sulphate in the presence of reducing agent at 37 degrees C extracted approximately 50% of the protein. Amino acid analysis and polyacrylamide gel electrophoresis demonstrated that the residual proteins were collagenous and of molecular weight. Extracted membranes retained their continuity of structure and exhibited a matrix composed of fibrous and globular elements when examined by electron microscopy. When the filtration properties of the membranes were studied in vitro they showed an enhanced capacity to retain proteins. The results suggest that basement membranes contain a matrix of fibrous proteins, which is responsible for the sieving characteristics of the native membranes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:volume
111
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
485-90
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
The effects of protein extraction on the structure and filtration properties of renal basement membranes.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.