Linked Life Data

7459332

Source:http://linkedlifedata.com/resource/pubmed/id/7459332

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
1981-4-13
pubmed:abstractText
The aqueous solution conformation of Arg-Lys-Asp-Val-Tyr (TP5), corresponding to positions 32-36 of the thymic hormone thymopoietin has been investigated by proton nuclear magnetic resonance (NMR). This pentapeptide fragment retains the biological activity of the parent protein, viz., induction of selective differentiation of T lymphocytes. All the observed NH and CH resonances of TP5 have been assigned, and the solution conformation of this peptide has been investigated by analysis of chemical shift variations with pH, vicinal NH-C alpha H coupling constant data, and amide hydrogen exchange rates. The latter were measured in H2O by using a combination technique consisting of the transfer of solvent saturation and saturation recovery NMR experiments. The data are compatible with the assumption of a highly motile dynamic equilibrium among different conformations for TP5. A comparison of the amide hydrogen exchange rates of the pentapeptide with that of solvated model compounds shows that Val4-NH is significantly shielded from the solvent. In addition, the chemical shift variations with pH suggest that the guanidino-N epsilon H of arginine is associated with one of the carboxylate groups. These observations provide specific boundary conditions for the construction of molecular models of the conformation(s) of TP5 in aqueous solution.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5557-63
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Solution conformation of thymopoietin32-36: a proton nuclear magnetic resonance study.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.