Linked Life Data

7457939

Source:http://linkedlifedata.com/resource/pubmed/id/7457939

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1981-3-27
pubmed:abstractText
The nature of the intensely fluorescent cells (IFC) in the livers of male Sprague-Dawley rats was evaluated using histochemical and pharmacological techniques. The number and distribution of IFC in portal areas were quantified using some of these techniques. Cells which were metachromatic as well as fluorescent for serotonin, histamine, and heparin were observed in the connective tissue of the portal space, hilus, and capsule of the liver. Cells with the characteristics of chromaffin, enterochromaffin, or enterochromaffin-like cells were not seen in these locations. Intravenous administration of compound 48/80 a known mast cell degranulator, caused a significant decrease in the number of fluorescent and metachromatic cells in the portal areas of the liver. However, no significant difference was found in the number of cells counted in either the 48/80 or control groups when comparing the data from several histochemical methods. These results provided evidence that: (1) IFC demonstrate both fluorescence and metachromasia and, therefore, are mast cells, (2) compound 48/80 causes a comparable decrease in the number of serotonin and histamine fluorescent as well as metachromatic cells, indicating concomitant rather than differential release of serotonin, histamine, heparin, and/or other metachromatic substances, and (3) each of the three histochemical methods appears of equivalent sensitivity when used to study the effect of various factors (i.e., 48/80) on the release of endogenous substances from IFC. The results of this study indicate that the effects of 48/80 in vivo might be mediated through the release of various vasoactive substances from these IFC (mast cells).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0003-276X
pubmed:author
pubmed:issnType
Print
pubmed:volume
198
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
475-84
pubmed:dateRevised
2009-11-3
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Characterization of intensely fluorescent cells in the liver of the rat. i. Histochemistry and 48/80-induced degranulation.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't