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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1981-3-24
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pubmed:abstractText |
Promastigotes of 4 Leishmania tropica isolates were biosynthetically labeled with 35S-methionine or surface radioiodinated, and the detergent lysates were analyzed by 2 dimensional gel electrophoresis. The protein patterns of cytoplasmic and membrane proteins detected in two independent isolates from simple cutaneous leishmaniasis cases were similar to each other, but were different from 2 isolates taken over a 20-yr interval from a case of leishmaniasis recidiva. The analysis of radioiodinated membrane proteins of L. tropica promastigotes revealed a simple pattern of 6 to 7 labeled major proteins and some minor ones. Unlike cytoplasmic proteins, membrane proteins were highly resistant to digestion by proteases. Partial cleavage of radioiodinated surface proteins by staphylococcal V-8 protease in the presence of 4.5 M urea and peptide mapping confirmed the presence of shared and isolate-specific proteins. The significance of these findings for the classification of Leishmania spp. and the pathogenesis of the different disease states that they cause are discussed.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
126
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
508-12
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7451987-Animals,
pubmed-meshheading:7451987-Antigens,
pubmed-meshheading:7451987-Chemical Precipitation,
pubmed-meshheading:7451987-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7451987-Humans,
pubmed-meshheading:7451987-Leishmaniasis,
pubmed-meshheading:7451987-Membrane Proteins,
pubmed-meshheading:7451987-Methionine,
pubmed-meshheading:7451987-Proteins,
pubmed-meshheading:7451987-Rabbits
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pubmed:year |
1981
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pubmed:articleTitle |
Identification and characterization of protein antigens of Leishmania tropica isolates.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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