Linked Life Data

7451433

Source:http://linkedlifedata.com/resource/pubmed/id/7451433

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-3-24
pubmed:abstractText
1-Alkyl-2-acetyl-sn-glycero-3-phosphocholine, a phospholipid with platelet activating and hypotensive properties, has an extremely labile acetate grouping. The acetate group is obviously important in the expression of the biological properties of this unique derivative of plasmanic acid since once it is hydrolyzed from the parent compound to form the lyso product, all biological activity is lost. Our studies show that the enzyme responsible for the hydrolysis of the acetate moiety, 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine: acetylhydrolase, occurs in the cytosolic fraction of a variety of tissues and has a pH optimum of 7.5 to 8.5. Effects of calcium, magnesium, EDTA, dithiothreitol, deoxycholate, and diisopropylfluorophosphate on the enzyme activity and the fact that egg phosphatidylcholine was not inhibitory indicate that acetylhydrolase activity has different properties from those normally associated with the phospholipase A2 that utilizes phospholipids with two long chain acyl groups. The highest specific activity of the acetylhydrolase occurred in kidney; lung and brain were also good sources of the enzyme. The soluble fraction from the kidney cortex had an apparent Km and Vmax of 3.1 microM and 17.8 nmol/min/mg of protein, respectively. Our results indicate that acetylhydrolase plays a significant role in the catabolism of 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
256
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
175-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
A specific acetylhydrolase for 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine (a hypotensive and platelet-activating lipid).
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.