rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
2
|
pubmed:dateCreated |
1981-3-27
|
pubmed:abstractText |
This paper presents a cytochemical study of a modified Schiff reagent in which 250 Tris-buffer has been incorporated in addition to 5 ml of N HCL and 1.0 g of potassium metabisulphite per 100 ml of an aqueous solution of 0.5% basic fuchsin. The results indicate production of an orange-coloured dye-reagent which when used on acid hydrolysed tissue sections reveals brilliantly stained nuclei. The shelf-life of this modified Tris-Schiff reagent is also much more than that of the conventional Schiff reagent. The in vitro absorption spectra of the orange-coloured dye-reagent together with those of the modified Tris-Schiff reagent with formalin as well as the conventional Schiff reagent with formalin are also presented. Apossible explanation for the increased shelf-life has been offered.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:issn |
0015-5586
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
18
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
129-32
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:7450603-Animals,
pubmed-meshheading:7450603-Cell Nucleus,
pubmed-meshheading:7450603-Histocytochemistry,
pubmed-meshheading:7450603-Indicators and Reagents,
pubmed-meshheading:7450603-Liver,
pubmed-meshheading:7450603-Periodic Acid-Schiff Reaction,
pubmed-meshheading:7450603-Rats,
pubmed-meshheading:7450603-Rosaniline Dyes,
pubmed-meshheading:7450603-Solutions,
pubmed-meshheading:7450603-Sulfites,
pubmed-meshheading:7450603-Tromethamine
|
pubmed:year |
1980
|
pubmed:articleTitle |
Cytochemical properties of the conventional Schiff reagent fortified with Tris-buffer.
|
pubmed:publicationType |
Journal Article,
In Vitro
|