Linked Life Data

7448203

Source:http://linkedlifedata.com/resource/pubmed/id/7448203

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-3-17
pubmed:abstractText
The metabolism of thyroxine, 3,3'-triiodothyronine and 3,3',5'-triiodothyronine was investigated in rat hepatoma cell cultures (R117-21B). These iodothyronines were labeled with 125I in the phenolic ring and the metabolites were analyzed by ion-exchange column chromatography. When thyroxine was incubated with the cells at 37 degrees C, its glucuronide was the major product and a little increase in 125I- was detected. Although 3,3',5-triiodothyronine was not observed in the incubation medium, this metabolite was clearly identified in the ethanol extract obtained from the cell homogenates after 24 h incubation. This cell line also metabolized labeled 3,3',5-triiodothyronine added to culture medium. After 24 h incubation, 3,3',5-triiodothyronine glucuronide was the major metabolite and iodothyronine sulfates were also formed. The sulfates contained, 3,3',5-triiodothyronine and 3,3'-diiodothyronine sulfates and an unknown component. In the metabolism of 3,3',5'-triiodothyronine, the cells were very active in carrying out glucuronidation and phenolic ring deiodination, and this metabolism yielded 3,3',5'-triiodothyronine and 3,3'-diiodothyronine glucuronides. The iodide fraction contained a small amount of 3,3'-diiodothyronine sulfate. These results show that the R117-21B rat hepatoma cells metabolize the thyroid hormones and their analogs by phenolic and nonphenolic ring deiodinations, by glucuronidation and by sulfation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
633
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
134-43
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Activation and inactivation of thyroxine by cultured rat hepatoma cells.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't