| pubmed-article:7443629 | pubmed:abstractText | A method for preparing reproducible chromatin fractions is described. Fragmentation is accomplished by expelling whole chromatin from an enclosed stainless-steel chamber through a restricted orifice utilizing low nitrogen pressure. Sheared chromatin is fractionated into two distinct heavy and light fractions by centrifugation on 5-20% linear sucrose gradient. | lld:pubmed |
